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Reaper-induced apoptosis in a vertebrate system.

Publication ,  Journal Article
Evans, EK; Kuwana, T; Strum, SL; Smith, JJ; Newmeyer, DD; Kornbluth, S
Published in: The EMBO journal
December 1997

The reaper protein of Drosophila melanogaster has been shown to be a central regulator of apoptosis in that organism. However, it has not been shown to function in any vertebrate nor have the cellular components required for its action been defined. In this report we show that reaper can induce rapid apoptosis in vitro using an apoptotic reconstitution system derived from Xenopus eggs. Moreover, we show that a subcellular fraction enriched in mitochondria is required for this process and that reaper, acting in conjunction with cytosolic factors, can trigger mitochondrial cytochrome c release. Bcl-2 antagonizes these effects, but high levels of reaper can overcome the Bcl-2 block. These results demonstrate that reaper can function in a vertebrate context, suggesting that reaper-responsive factors are conserved elements of the apoptotic program.

Duke Scholars

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

December 1997

Volume

16

Issue

24

Start / End Page

7372 / 7381

Related Subject Headings

  • Xenopus
  • Vertebrates
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Peptides
  • Oocytes
  • Mitochondria
  • Kinetics
  • Female
  • Drosophila melanogaster
 

Citation

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Evans, E. K., Kuwana, T., Strum, S. L., Smith, J. J., Newmeyer, D. D., & Kornbluth, S. (1997). Reaper-induced apoptosis in a vertebrate system. The EMBO Journal, 16(24), 7372–7381. https://doi.org/10.1093/emboj/16.24.7372
Evans, E. K., T. Kuwana, S. L. Strum, J. J. Smith, D. D. Newmeyer, and S. Kornbluth. “Reaper-induced apoptosis in a vertebrate system.The EMBO Journal 16, no. 24 (December 1997): 7372–81. https://doi.org/10.1093/emboj/16.24.7372.
Evans EK, Kuwana T, Strum SL, Smith JJ, Newmeyer DD, Kornbluth S. Reaper-induced apoptosis in a vertebrate system. The EMBO journal. 1997 Dec;16(24):7372–81.
Evans, E. K., et al. “Reaper-induced apoptosis in a vertebrate system.The EMBO Journal, vol. 16, no. 24, Dec. 1997, pp. 7372–81. Epmc, doi:10.1093/emboj/16.24.7372.
Evans EK, Kuwana T, Strum SL, Smith JJ, Newmeyer DD, Kornbluth S. Reaper-induced apoptosis in a vertebrate system. The EMBO journal. 1997 Dec;16(24):7372–7381.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

December 1997

Volume

16

Issue

24

Start / End Page

7372 / 7381

Related Subject Headings

  • Xenopus
  • Vertebrates
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Peptides
  • Oocytes
  • Mitochondria
  • Kinetics
  • Female
  • Drosophila melanogaster