Skip to main content

Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.

Publication ,  Journal Article
Kornbluth, S; Dasso, M; Newport, J
Published in: The Journal of cell biology
May 1994

TC4, a ras-like G protein, has been implicated in the feedback pathway linking the onset of mitosis to the completion of DNA replication. In this report we find distinct roles for TC4 in both nuclear assembly and cell cycle progression. Mutant and wild-type forms of TC4 were added to Xenopus egg extracts capable of assembling nuclei around chromatin templates in vitro. We found that a mutant TC4 protein defective in GTP binding (GDP-bound form) suppressed nuclear growth and prevented DNA replication. Nuclear transport under these conditions approximated normal levels. In a separate set of experiments using a cell-free extract of Xenopus eggs that cycles between S and M phases, the GDP-bound form of TC4 had dramatic effects, blocking entry into mitosis even in the complete absence of nuclei. The effect of this mutant TC4 protein on cell cycle progression is mediated by phosphorylation of p34cdc2 on tyrosine and threonine residues, negatively regulating cdc2 kinase activity. Therefore, we provide direct biochemical evidence for a role of TC4 in both maintaining nuclear structure and in the signaling pathways that regulate entry into mitosis.

Duke Scholars

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

May 1994

Volume

125

Issue

4

Start / End Page

705 / 719

Related Subject Headings

  • ran GTP-Binding Protein
  • Xenopus
  • Signal Transduction
  • Nuclear Proteins
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Humans
  • Guanosine Triphosphate
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kornbluth, S., Dasso, M., & Newport, J. (1994). Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. The Journal of Cell Biology, 125(4), 705–719. https://doi.org/10.1083/jcb.125.4.705
Kornbluth, S., M. Dasso, and J. Newport. “Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.The Journal of Cell Biology 125, no. 4 (May 1994): 705–19. https://doi.org/10.1083/jcb.125.4.705.
Kornbluth S, Dasso M, Newport J. Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. The Journal of cell biology. 1994 May;125(4):705–19.
Kornbluth, S., et al. “Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression.The Journal of Cell Biology, vol. 125, no. 4, May 1994, pp. 705–19. Epmc, doi:10.1083/jcb.125.4.705.
Kornbluth S, Dasso M, Newport J. Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. The Journal of cell biology. 1994 May;125(4):705–719.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

May 1994

Volume

125

Issue

4

Start / End Page

705 / 719

Related Subject Headings

  • ran GTP-Binding Protein
  • Xenopus
  • Signal Transduction
  • Nuclear Proteins
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Humans
  • Guanosine Triphosphate
  • Developmental Biology