Skip to main content

RCC1, a regulator of mitosis, is essential for DNA replication.

Publication ,  Journal Article
Dasso, M; Nishitani, H; Kornbluth, S; Nishimoto, T; Newport, JW
Published in: Molecular and cellular biology
August 1992

Temperature-sensitive mutants in the RCC1 gene of BHK cells fail to maintain a correct temporal order of the cell cycle and will prematurely condense their chromosomes and enter mitosis at the restrictive temperature without having completed S phase. We have used Xenopus egg extracts to investigate the role that RCC1 plays in interphase nuclear functions and how this role might contribute to the known phenotype of temperature-sensitive RCC1 mutants. By immunodepleting RCC1 protein from egg extracts, we find that it is required for neither chromatin decondensation nor nuclear formation but that it is absolutely required for the replication of added sperm chromatin DNA. Our results further suggest that RCC1 does not participate enzymatically in replication but may be part of a structural complex which is required for the formation or maintenance of the replication machinery. By disrupting the replication complex, the loss of RCC1 might lead directly to disruption of the regulatory system which prevents the initiation of mitosis before the completion of DNA replication.

Duke Scholars

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

August 1992

Volume

12

Issue

8

Start / End Page

3337 / 3345

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Proteins
  • Plasmids
  • Oocytes
  • Nucleosomes
  • Nuclear Proteins
  • Mutation
  • Mitosis
  • Kinetics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dasso, M., Nishitani, H., Kornbluth, S., Nishimoto, T., & Newport, J. W. (1992). RCC1, a regulator of mitosis, is essential for DNA replication. Molecular and Cellular Biology, 12(8), 3337–3345. https://doi.org/10.1128/mcb.12.8.3337-3345.1992
Dasso, M., H. Nishitani, S. Kornbluth, T. Nishimoto, and J. W. Newport. “RCC1, a regulator of mitosis, is essential for DNA replication.Molecular and Cellular Biology 12, no. 8 (August 1992): 3337–45. https://doi.org/10.1128/mcb.12.8.3337-3345.1992.
Dasso M, Nishitani H, Kornbluth S, Nishimoto T, Newport JW. RCC1, a regulator of mitosis, is essential for DNA replication. Molecular and cellular biology. 1992 Aug;12(8):3337–45.
Dasso, M., et al. “RCC1, a regulator of mitosis, is essential for DNA replication.Molecular and Cellular Biology, vol. 12, no. 8, Aug. 1992, pp. 3337–45. Epmc, doi:10.1128/mcb.12.8.3337-3345.1992.
Dasso M, Nishitani H, Kornbluth S, Nishimoto T, Newport JW. RCC1, a regulator of mitosis, is essential for DNA replication. Molecular and cellular biology. 1992 Aug;12(8):3337–3345.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

August 1992

Volume

12

Issue

8

Start / End Page

3337 / 3345

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Proteins
  • Plasmids
  • Oocytes
  • Nucleosomes
  • Nuclear Proteins
  • Mutation
  • Mitosis
  • Kinetics