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In vitro cell cycle arrest induced by using artificial DNA templates.

Publication ,  Journal Article
Kornbluth, S; Smythe, C; Newport, JW
Published in: Molecular and cellular biology
July 1992

In cell extracts of Xenopus eggs which oscillate between S and M phases of the cell cycle, the onset of mitosis is blocked by the presence of incompletely replicated DNA. In this report, we show that several artificial DNA templates (M13 single-stranded DNA and double-stranded plasmid DNA) can trigger this feedback pathway, which inhibits mitosis. Single-stranded M13 DNA is much more effective than double-stranded plasmid DNA at inhibiting the onset of mitosis. Furthermore, we have shown that low levels of M13 single-stranded DNA and high levels of double-stranded plasmid DNA can elevate the tyrosine kinase activity responsible for phosphorylating p34cdc2, thereby inactivating maturation-promoting factor and inhibiting entry into mitosis. This constitutes a simplified system with which to study the signal transduction pathway from the DNA template to the tyrosine kinase responsible for inhibiting p34cdc2 activity.

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Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

July 1992

Volume

12

Issue

7

Start / End Page

3216 / 3223

Related Subject Headings

  • Xenopus
  • Signal Transduction
  • Protein Kinases
  • Protamine Kinase
  • Plasmids
  • Phosphorylation
  • Mitosis
  • Maturation-Promoting Factor
  • Enzyme Activation
  • Developmental Biology
 

Citation

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Kornbluth, S., Smythe, C., & Newport, J. W. (1992). In vitro cell cycle arrest induced by using artificial DNA templates. Molecular and Cellular Biology, 12(7), 3216–3223. https://doi.org/10.1128/mcb.12.7.3216-3223.1992
Kornbluth, S., C. Smythe, and J. W. Newport. “In vitro cell cycle arrest induced by using artificial DNA templates.Molecular and Cellular Biology 12, no. 7 (July 1992): 3216–23. https://doi.org/10.1128/mcb.12.7.3216-3223.1992.
Kornbluth S, Smythe C, Newport JW. In vitro cell cycle arrest induced by using artificial DNA templates. Molecular and cellular biology. 1992 Jul;12(7):3216–23.
Kornbluth, S., et al. “In vitro cell cycle arrest induced by using artificial DNA templates.Molecular and Cellular Biology, vol. 12, no. 7, July 1992, pp. 3216–23. Epmc, doi:10.1128/mcb.12.7.3216-3223.1992.
Kornbluth S, Smythe C, Newport JW. In vitro cell cycle arrest induced by using artificial DNA templates. Molecular and cellular biology. 1992 Jul;12(7):3216–3223.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

July 1992

Volume

12

Issue

7

Start / End Page

3216 / 3223

Related Subject Headings

  • Xenopus
  • Signal Transduction
  • Protein Kinases
  • Protamine Kinase
  • Plasmids
  • Phosphorylation
  • Mitosis
  • Maturation-Promoting Factor
  • Enzyme Activation
  • Developmental Biology