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Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.

Publication ,  Journal Article
Mansukhani, A; Dell'Era, P; Moscatelli, D; Kornbluth, S; Hanafusa, H; Basilico, C
Published in: Proceedings of the National Academy of Sciences of the United States of America
April 1992

The bek gene encodes a member of the high-affinity fibroblast growth factor receptor family. The BEK/FGFR-2 receptor is a membrane-spanning tyrosine kinase with the typical features of FGF receptors. We have cloned a murine bek cDNA and expressed it in receptor-negative Chinese hamster ovary cells and in 32D myeloid cells. The BEK receptor expressed in Chinese hamster ovary cells binds acidic FGF, basic FGF, and Kaposi FGF equally well but does not bind keratinocyte growth factor or FGF-5 appreciably. Upon treatment with basic FGF or Kaposi FGF, the BEK receptor is phosphorylated and a mitogenic response is achieved. Heparan sulfate proteoglycans have been shown to play an obligate role in basic FGF binding to the high-affinity FLG receptor. Unlike the BEK-expressing Chinese hamster ovary cells, 32D cells expressing the BEK receptor require the addition of exogenous heparin in order to grow in the presence of basic FGF or Kaposi FGF. We show that the addition of heparin greatly enhances the binding of radio-labeled basic FGF to the receptor. Thus the BEK receptor, like FLG, also requires an interaction with heparan sulfate proteoglycans to facilitate binding to its ligands.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

April 1992

Volume

89

Issue

8

Start / End Page

3305 / 3309

Related Subject Headings

  • Transfection
  • Receptors, Fibroblast Growth Factor
  • Receptors, Cell Surface
  • Protein-Tyrosine Kinases
  • Molecular Sequence Data
  • Male
  • Kinetics
  • Heparin
  • Gene Library
  • Fibroblast Growth Factors
 

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Mansukhani, A., Dell’Era, P., Moscatelli, D., Kornbluth, S., Hanafusa, H., & Basilico, C. (1992). Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin. Proceedings of the National Academy of Sciences of the United States of America, 89(8), 3305–3309. https://doi.org/10.1073/pnas.89.8.3305
Mansukhani, A., P. Dell’Era, D. Moscatelli, S. Kornbluth, H. Hanafusa, and C. Basilico. “Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.Proceedings of the National Academy of Sciences of the United States of America 89, no. 8 (April 1992): 3305–9. https://doi.org/10.1073/pnas.89.8.3305.
Mansukhani A, Dell’Era P, Moscatelli D, Kornbluth S, Hanafusa H, Basilico C. Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin. Proceedings of the National Academy of Sciences of the United States of America. 1992 Apr;89(8):3305–9.
Mansukhani, A., et al. “Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 8, Apr. 1992, pp. 3305–09. Epmc, doi:10.1073/pnas.89.8.3305.
Mansukhani A, Dell’Era P, Moscatelli D, Kornbluth S, Hanafusa H, Basilico C. Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin. Proceedings of the National Academy of Sciences of the United States of America. 1992 Apr;89(8):3305–3309.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

April 1992

Volume

89

Issue

8

Start / End Page

3305 / 3309

Related Subject Headings

  • Transfection
  • Receptors, Fibroblast Growth Factor
  • Receptors, Cell Surface
  • Protein-Tyrosine Kinases
  • Molecular Sequence Data
  • Male
  • Kinetics
  • Heparin
  • Gene Library
  • Fibroblast Growth Factors