Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.
A number of mutants of polyomavirus middle T antigen (MTag) were constructed into replication-competent avian retroviruses. To assess the ability of these MTag variants to transform and to associate with the avian p60c-src and p62c-yes proto-oncogene products, we used these viruses to infect chicken embryo fibroblasts. We found that the ability of individual mutant MTags to associate with p62c-yes correlated well with the ability of these mutants to transform, as has been previously shown for the association of MTag with p60c-src. All transformation-competent mutant MTags retained the ability to complex with p62c-yes. Two transformation-defective mutants, RX67 and RX68, which could weakly associate with p60c-src, were unable to associate with p62c-yes.dl1015, a transformation-defective mutant which could associate with p60c-src and with a phosphatidylinositol kinase activity, was also able to associate with p62c-yes. Therefore, some as yet unmeasured biochemical property is defective in this mutant.
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Related Subject Headings
- src-Family Kinases
- Virology
- Transfection
- Proto-Oncogene Proteins pp60(c-src)
- Proto-Oncogene Proteins c-yes
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Protein Binding
- Polyomavirus
- Plasmids
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- src-Family Kinases
- Virology
- Transfection
- Proto-Oncogene Proteins pp60(c-src)
- Proto-Oncogene Proteins c-yes
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Protein Binding
- Polyomavirus
- Plasmids