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Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.

Publication ,  Journal Article
Kornbluth, S; Cheng, SH; Markland, W; Fukui, Y; Hanafusa, H
Published in: Journal of virology
April 1990

A number of mutants of polyomavirus middle T antigen (MTag) were constructed into replication-competent avian retroviruses. To assess the ability of these MTag variants to transform and to associate with the avian p60c-src and p62c-yes proto-oncogene products, we used these viruses to infect chicken embryo fibroblasts. We found that the ability of individual mutant MTags to associate with p62c-yes correlated well with the ability of these mutants to transform, as has been previously shown for the association of MTag with p60c-src. All transformation-competent mutant MTags retained the ability to complex with p62c-yes. Two transformation-defective mutants, RX67 and RX68, which could weakly associate with p60c-src, were unable to associate with p62c-yes.dl1015, a transformation-defective mutant which could associate with p60c-src and with a phosphatidylinositol kinase activity, was also able to associate with p62c-yes. Therefore, some as yet unmeasured biochemical property is defective in this mutant.

Duke Scholars

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

April 1990

Volume

64

Issue

4

Start / End Page

1584 / 1589

Related Subject Headings

  • src-Family Kinases
  • Virology
  • Transfection
  • Proto-Oncogene Proteins pp60(c-src)
  • Proto-Oncogene Proteins c-yes
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Binding
  • Polyomavirus
  • Plasmids
 

Citation

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Kornbluth, S., Cheng, S. H., Markland, W., Fukui, Y., & Hanafusa, H. (1990). Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability. Journal of Virology, 64(4), 1584–1589. https://doi.org/10.1128/jvi.64.4.1584-1589.1990
Kornbluth, S., S. H. Cheng, W. Markland, Y. Fukui, and H. Hanafusa. “Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.Journal of Virology 64, no. 4 (April 1990): 1584–89. https://doi.org/10.1128/jvi.64.4.1584-1589.1990.
Kornbluth S, Cheng SH, Markland W, Fukui Y, Hanafusa H. Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability. Journal of virology. 1990 Apr;64(4):1584–9.
Kornbluth, S., et al. “Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability.Journal of Virology, vol. 64, no. 4, Apr. 1990, pp. 1584–89. Epmc, doi:10.1128/jvi.64.4.1584-1589.1990.
Kornbluth S, Cheng SH, Markland W, Fukui Y, Hanafusa H. Association of p62c-yes with polyomavirus middle T-antigen mutants correlates with transforming ability. Journal of virology. 1990 Apr;64(4):1584–1589.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

April 1990

Volume

64

Issue

4

Start / End Page

1584 / 1589

Related Subject Headings

  • src-Family Kinases
  • Virology
  • Transfection
  • Proto-Oncogene Proteins pp60(c-src)
  • Proto-Oncogene Proteins c-yes
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Binding
  • Polyomavirus
  • Plasmids