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Characterization of avian and viral p60src proteins expressed in yeast.

Publication ,  Journal Article
Kornbluth, S; Jove, R; Hanafusa, H
Published in: Proceedings of the National Academy of Sciences of the United States of America
July 1987

Avian and viral p60src proteins were expressed from a galactose-inducible promoter in the yeast Saccharomyces cerevisiae. Both the viral and cellular src proteins produced in yeast cells were myristoylated at their amino termini, as is the case for src proteins expressed in chicken embryo fibroblasts. The viral src protein produced in yeast autophosphorylated at tyrosine-416 in vivo and had approximately the same level of in vitro kinase activity as p60v-src expressed in Rous sarcoma virus-transformed cells. Unlike p60c-src expressed in chicken cells, which is phosphorylated on tyrosine in vivo almost exclusively at tyrosine-527, p60c-src expressed in yeast was phosphorylated 2.5-3 times more at tyrosine-416 than at tyrosine-527. The specific activity of the p60c-src produced in yeast was 2.5-5.0 times higher than that of p60c-src overexpressed from a retroviral vector in chicken cells, implicating the altered state of in vivo phosphorylation in modulation of the in vitro kinase activity. The expression of p60v-src substantially slowed down the growth of the yeast cells, suggesting that phosphorylation of yeast proteins essential for cell growth may have interfered with their proper functioning.

Duke Scholars

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

July 1987

Volume

84

Issue

13

Start / End Page

4455 / 4459

Related Subject Headings

  • Tyrosine
  • Saccharomyces cerevisiae
  • Retroviridae Proteins
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins pp60(c-src)
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Processing, Post-Translational
  • Phosphotyrosine
 

Citation

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Kornbluth, S., Jove, R., & Hanafusa, H. (1987). Characterization of avian and viral p60src proteins expressed in yeast. Proceedings of the National Academy of Sciences of the United States of America, 84(13), 4455–4459. https://doi.org/10.1073/pnas.84.13.4455
Kornbluth, S., R. Jove, and H. Hanafusa. “Characterization of avian and viral p60src proteins expressed in yeast.Proceedings of the National Academy of Sciences of the United States of America 84, no. 13 (July 1987): 4455–59. https://doi.org/10.1073/pnas.84.13.4455.
Kornbluth S, Jove R, Hanafusa H. Characterization of avian and viral p60src proteins expressed in yeast. Proceedings of the National Academy of Sciences of the United States of America. 1987 Jul;84(13):4455–9.
Kornbluth, S., et al. “Characterization of avian and viral p60src proteins expressed in yeast.Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 13, July 1987, pp. 4455–59. Epmc, doi:10.1073/pnas.84.13.4455.
Kornbluth S, Jove R, Hanafusa H. Characterization of avian and viral p60src proteins expressed in yeast. Proceedings of the National Academy of Sciences of the United States of America. 1987 Jul;84(13):4455–4459.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

July 1987

Volume

84

Issue

13

Start / End Page

4455 / 4459

Related Subject Headings

  • Tyrosine
  • Saccharomyces cerevisiae
  • Retroviridae Proteins
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins pp60(c-src)
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Processing, Post-Translational
  • Phosphotyrosine