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Association of the polyomavirus middle-T antigen with c-yes protein.

Publication ,  Journal Article
Kornbluth, S; Sudol, M; Hanafusa, H
Published in: Nature
January 1987

Expression of the middle-T antigen of polyomavirus is sufficient to induce transformation of fibroblasts in culture and tumour formation in whole animals. Middle-T can form a complex with the cellular src gene product (p60c-src) and can be phosphorylated by p60c-src in vitro. Studies using middle-T mutants have suggested that the association of middle-T with p60c-src may be necessary but not sufficient for transformation. Therefore, we addressed the possibility that middle-T could interact with other tyrosine protein kinases structurally related to p60c-src. Using antibody raised against a fusion protein between beta-galactosidase and amino-terminal sequences of p90gag-yes from Y73 virus (anti-yes antibody), we have found that middle-T can associate with and be phosphorylated by the c-yes proto-oncogene product, a protein of relative molecular mass (Mr) 62,000 (62K). This raises the possibility that the middle-T-p62c-yes complex contributes to transformation by polyomavirus.

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Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

January 1987

Volume

325

Issue

7000

Start / End Page

171 / 173

Related Subject Headings

  • Viral Proteins
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Binding
  • Polyomavirus
  • Phosphoproteins
  • General Science & Technology
  • Chemical Precipitation
  • Cell Transformation, Viral
  • Antigens, Viral, Tumor
 

Citation

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Kornbluth, S., Sudol, M., & Hanafusa, H. (1987). Association of the polyomavirus middle-T antigen with c-yes protein. Nature, 325(7000), 171–173. https://doi.org/10.1038/325171a0
Kornbluth, S., M. Sudol, and H. Hanafusa. “Association of the polyomavirus middle-T antigen with c-yes protein.Nature 325, no. 7000 (January 1987): 171–73. https://doi.org/10.1038/325171a0.
Kornbluth S, Sudol M, Hanafusa H. Association of the polyomavirus middle-T antigen with c-yes protein. Nature. 1987 Jan;325(7000):171–3.
Kornbluth, S., et al. “Association of the polyomavirus middle-T antigen with c-yes protein.Nature, vol. 325, no. 7000, Jan. 1987, pp. 171–73. Epmc, doi:10.1038/325171a0.
Kornbluth S, Sudol M, Hanafusa H. Association of the polyomavirus middle-T antigen with c-yes protein. Nature. 1987 Jan;325(7000):171–173.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

January 1987

Volume

325

Issue

7000

Start / End Page

171 / 173

Related Subject Headings

  • Viral Proteins
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Protein Binding
  • Polyomavirus
  • Phosphoproteins
  • General Science & Technology
  • Chemical Precipitation
  • Cell Transformation, Viral
  • Antigens, Viral, Tumor