Skip to main content

Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility.

Publication ,  Journal Article
Kondilis-Mangum, HD; Shih, H-Y; Mahowald, G; Sleckman, BP; Krangel, MS
Published in: J Immunol
December 15, 2011

Ag receptor loci are regulated to promote allelic exclusion, but the mechanisms are not well understood. Assembly of a functional TCR β-chain gene triggers feedback inhibition of V(β)-to-DJ(β) recombination in double-positive (DP) thymocytes, which correlates with reduced V(β) chromatin accessibility and a locus conformational change that separates V(β) from DJ(β) gene segments. We previously generated a Tcrb allele that maintained V(β) accessibility but was still subject to feedback inhibition in DP thymocytes. We have now further analyzed the contributions of chromatin accessibility and locus conformation to feedback inhibition using two novel TCR alleles. We show that reduced V(β) accessibility and increased distance between V(β) and DJ(β) gene segments both enforce feedback inhibition in DP thymocytes.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

December 15, 2011

Volume

187

Issue

12

Start / End Page

6374 / 6381

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleic Acid Conformation
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, 129 Strain
  • Mice
  • Membrane Glycoproteins
  • Immunology
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kondilis-Mangum, H. D., Shih, H.-Y., Mahowald, G., Sleckman, B. P., & Krangel, M. S. (2011). Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility. J Immunol, 187(12), 6374–6381. https://doi.org/10.4049/jimmunol.1102611
Kondilis-Mangum, Hrisavgi D., Han-Yu Shih, Grace Mahowald, Barry P. Sleckman, and Michael S. Krangel. “Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility.J Immunol 187, no. 12 (December 15, 2011): 6374–81. https://doi.org/10.4049/jimmunol.1102611.
Kondilis-Mangum HD, Shih H-Y, Mahowald G, Sleckman BP, Krangel MS. Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility. J Immunol. 2011 Dec 15;187(12):6374–81.
Kondilis-Mangum, Hrisavgi D., et al. “Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility.J Immunol, vol. 187, no. 12, Dec. 2011, pp. 6374–81. Pubmed, doi:10.4049/jimmunol.1102611.
Kondilis-Mangum HD, Shih H-Y, Mahowald G, Sleckman BP, Krangel MS. Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility. J Immunol. 2011 Dec 15;187(12):6374–6381.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

December 15, 2011

Volume

187

Issue

12

Start / End Page

6374 / 6381

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleic Acid Conformation
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, 129 Strain
  • Mice
  • Membrane Glycoproteins
  • Immunology
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor