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Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains.

Publication ,  Journal Article
Shingai, M; Donau, OK; Schmidt, SD; Gautam, R; Plishka, RJ; Buckler-White, A; Sadjadpour, R; Lee, WR; LaBranche, CC; Montefiori, DC; Martin, MA ...
Published in: Proc Natl Acad Sci U S A
November 27, 2012

The induction of broadly reacting neutralizing antibodies has been a major goal of HIV vaccine research. Characterization of a pathogenic CCR5 (R5)-tropic SIV/HIV chimeric virus (SHIV) molecular clone (SHIV(AD8-EO)) revealed that eight of eight infected animals developed cross-reactive neutralizing antibodies (NAbs) directed against an envelope glycoprotein derived from the heterologous HIV-1(DH12) strain. A panel of plasmas, collected from monkeys inoculated with either molecularly cloned or uncloned SHIV(AD8) stocks, exhibited cross-neutralization against multiple tier 1 and tier 2 HIV-1 clade B isolates. One SHIV(AD8)-infected animal also developed NAbs against clades A and C HIV-1 strains. In this particular infected macaque, the cross-reacting anti-HIV-1 NAbs produced between weeks 7 and 13 were directed against a neutralization-sensitive virus strain, whereas neutralizing activities emerging at weeks 41-51 targeted more neutralization-resistant HIV-1 isolates. These results indicate that the SHIV(AD8) macaque model represents a potentially valuable experimental system for investigating B-cell maturation and the induction of cross-reactive NAbs directed against multiple HIV-1 strains.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 27, 2012

Volume

109

Issue

48

Start / End Page

19769 / 19774

Location

United States

Related Subject Headings

  • Simian Acquired Immunodeficiency Syndrome
  • Receptors, CCR5
  • Macaca mulatta
  • Immunophenotyping
  • HIV-1
  • Cross Reactions
  • Antibodies, Neutralizing
  • Animals
 

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Shingai, M., Donau, O. K., Schmidt, S. D., Gautam, R., Plishka, R. J., Buckler-White, A., … Martin, M. A. (2012). Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains. Proc Natl Acad Sci U S A, 109(48), 19769–19774. https://doi.org/10.1073/pnas.1217443109
Shingai, Masashi, Olivia K. Donau, Stephen D. Schmidt, Rajeev Gautam, Ronald J. Plishka, Alicia Buckler-White, Reza Sadjadpour, et al. “Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains.Proc Natl Acad Sci U S A 109, no. 48 (November 27, 2012): 19769–74. https://doi.org/10.1073/pnas.1217443109.
Shingai M, Donau OK, Schmidt SD, Gautam R, Plishka RJ, Buckler-White A, et al. Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19769–74.
Shingai, Masashi, et al. “Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains.Proc Natl Acad Sci U S A, vol. 109, no. 48, Nov. 2012, pp. 19769–74. Pubmed, doi:10.1073/pnas.1217443109.
Shingai M, Donau OK, Schmidt SD, Gautam R, Plishka RJ, Buckler-White A, Sadjadpour R, Lee WR, LaBranche CC, Montefiori DC, Mascola JR, Nishimura Y, Martin MA. Most rhesus macaques infected with the CCR5-tropic SHIV(AD8) generate cross-reactive antibodies that neutralize multiple HIV-1 strains. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19769–19774.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 27, 2012

Volume

109

Issue

48

Start / End Page

19769 / 19774

Location

United States

Related Subject Headings

  • Simian Acquired Immunodeficiency Syndrome
  • Receptors, CCR5
  • Macaca mulatta
  • Immunophenotyping
  • HIV-1
  • Cross Reactions
  • Antibodies, Neutralizing
  • Animals