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Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance.

Publication ,  Journal Article
Bennett, WA; Lagoo-Deenadayalan, S; Whitworth, NS; Brackin, MN; Hale, E; Cowan, BD
Published in: Early Pregnancy
September 1997

Interleukin-10 (IL-10) is a T-helper type-2 (Th2) cytokine noted for its ability to suppress cytokine synthesis by T-helper type-1 (Th1) cells. IL-10 may play a role in pregnancy immunotolerance through the establishment of a Th2 cytokine bias at the maternal-fetal interface. This study examines the expression and production of IL-10 by normal and malignant human trophoblast. Term placental biopsies, cloned choriocarcinoma cell lines and isolated human trophoblast were utilized for the study of IL-10 expression. Choriocarcinoma cells (BeWo, JEG-3, JAR) were maintained in T-flask culture until confluence and then harvested by enzymatic dispersion. Purified term trophoblast were obtained by sequential trypsin/DNAse digests and CD9 immunoaffinity chromatography. Amplified IL-10 mRNA was detected by a reverse transcriptase polymerase chain reaction (RTPCR) technique. BeWo cells were maintained in artificial capillary culture (ACC) and conditioned media assayed for IL-10. Granulocyte macrophage-colony stimulating factor (GM-CSF; 1.0, 10.0 and 100.0 ng/ml) was added to the BeWo cultures to examine its effects on trophoblast IL-10 production. IL-10 determinations were performed using a human ELISA system. IL-10 mRNA was detected in each trophoblast cell type examined with the exception of the JEG-3 choriocarcinoma cell line. IL-10 protein was also detected (range 6-22 pg/ml) in BeWo media on days 8 to 11 of culture. When serum was reduced in the culture media, IL-10 levels fell below the sensitivity of the assay (5 pg/ml). Subsequent addition of GM-CSF stimulated BeWo IL-10 secretion in a dose-related fashion. These results support the concept IL-10 is expressed at the human maternal-fetal interface, and production of this important immunoregulatory molecule may be regulated, in part, by GM-CSF.

Duke Scholars

Published In

Early Pregnancy

ISSN

1354-4195

Publication Date

September 1997

Volume

3

Issue

3

Start / End Page

190 / 198

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trophoblasts
  • Reverse Transcriptase Polymerase Chain Reaction
  • Radioimmunoassay
  • RNA, Messenger
  • Pregnancy
  • Placenta
  • Interleukin-10
  • Immune Tolerance
  • Humans
 

Citation

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Bennett, W. A., Lagoo-Deenadayalan, S., Whitworth, N. S., Brackin, M. N., Hale, E., & Cowan, B. D. (1997). Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance. Early Pregnancy, 3(3), 190–198.
Bennett, W. A., S. Lagoo-Deenadayalan, N. S. Whitworth, M. N. Brackin, E. Hale, and B. D. Cowan. “Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance.Early Pregnancy 3, no. 3 (September 1997): 190–98.
Bennett WA, Lagoo-Deenadayalan S, Whitworth NS, Brackin MN, Hale E, Cowan BD. Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance. Early Pregnancy. 1997 Sep;3(3):190–8.
Bennett, W. A., et al. “Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance.Early Pregnancy, vol. 3, no. 3, Sept. 1997, pp. 190–98.
Bennett WA, Lagoo-Deenadayalan S, Whitworth NS, Brackin MN, Hale E, Cowan BD. Expression and production of interleukin-10 by human trophoblast: relationship to pregnancy immunotolerance. Early Pregnancy. 1997 Sep;3(3):190–198.

Published In

Early Pregnancy

ISSN

1354-4195

Publication Date

September 1997

Volume

3

Issue

3

Start / End Page

190 / 198

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trophoblasts
  • Reverse Transcriptase Polymerase Chain Reaction
  • Radioimmunoassay
  • RNA, Messenger
  • Pregnancy
  • Placenta
  • Interleukin-10
  • Immune Tolerance
  • Humans