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Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane.

Publication ,  Journal Article
Lee, S-Y; Lee, A; Chen, J; MacKinnon, R
Published in: Proc Natl Acad Sci U S A
October 25, 2005

Voltage-dependent ion channels gate open in response to changes in cell membrane voltage. This form of gating permits the propagation of action potentials. We present two structures of the voltage-dependent K(+) channel KvAP, in complex with monoclonal Fv fragments (3.9 A) and without antibody fragments (8 A). We also studied KvAP with disulfide cross-bridges in lipid membranes. Analyzing these data in the context of the crystal structure of Kv1.2 and EPR data on KvAP we reach the following conclusions: (i) KvAP is similar in structure to Kv1.2 with a very modest difference in the orientation of its voltage sensor; (ii) mAb fragments are not the source of non-native conformations of KvAP in crystal structures; (iii) because KvAP contains separate loosely adherent domains, a lipid membrane is required to maintain their correct relative orientations, and (iv) the model of KvAP is consistent with the proposal of voltage sensing through the movement of an arginine-containing helix-turn-helix element at the protein-lipid interface.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 25, 2005

Volume

102

Issue

43

Start / End Page

15441 / 15446

Location

United States

Related Subject Headings

  • Protein Conformation
  • Potassium Channels, Voltage-Gated
  • Membrane Lipids
  • Lipid Bilayers
  • Kv1.2 Potassium Channel
  • Crystallization
 

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Lee, S.-Y., Lee, A., Chen, J., & MacKinnon, R. (2005). Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. Proc Natl Acad Sci U S A, 102(43), 15441–15446. https://doi.org/10.1073/pnas.0507651102
Lee, Seok-Yong, Alice Lee, Jiayun Chen, and Roderick MacKinnon. “Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane.Proc Natl Acad Sci U S A 102, no. 43 (October 25, 2005): 15441–46. https://doi.org/10.1073/pnas.0507651102.
Lee S-Y, Lee A, Chen J, MacKinnon R. Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15441–6.
Lee, Seok-Yong, et al. “Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane.Proc Natl Acad Sci U S A, vol. 102, no. 43, Oct. 2005, pp. 15441–46. Pubmed, doi:10.1073/pnas.0507651102.
Lee S-Y, Lee A, Chen J, MacKinnon R. Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15441–15446.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 25, 2005

Volume

102

Issue

43

Start / End Page

15441 / 15446

Location

United States

Related Subject Headings

  • Protein Conformation
  • Potassium Channels, Voltage-Gated
  • Membrane Lipids
  • Lipid Bilayers
  • Kv1.2 Potassium Channel
  • Crystallization