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Combining 'caged-dopamine' photolysis with fast-scan cyclic voltammetry to assess dopamine clearance and release autoinhibition in vitro.

Publication ,  Journal Article
Lee, TH; Gee, KR; Ellinwood, EH; Seidler, FJ
Published in: J Neurosci Methods
August 1996

We have developed a methodology for inducing a rapid rise in extracellular dopamine concentrations. The clearance of the applied dopamine, as well as its effect on the endogenous dopamine release (i.e., autoinhibition), was then examined using fast scan cyclic voltammetry. In a recording chamber mounted on a Nikon Optiphot epifluorescence microscope, coronal rat brain slices containing either the caudate nucleus or prefrontal cortex were perfused with ACSF containing 100-200 microM 'caged-DA.' UV illumination (100-200 ms) focused at the tip of the recording electrode produced a peak DA concentration of 1-2 microM within 100-200 ms of terminating the illumination. The caudate nucleus exhibited a faster clearance rate for photo-released DA compared to the prefrontal cortex. Cocaine reduced the clearance rates in both the caudate nucleus and prefrontal cortex. In the prefrontal cortex a combination of desipramine/clomipramine also reduced dopamine clearance, suggesting heterologous uptake of the applied DA by noradrenergic and/or serotonergic terminals. Photo-released dopamine inhibited release of endogenous caudate DA release evoked by single electrical stimulation. The advantages of this methodology are discussed.

Duke Scholars

Published In

J Neurosci Methods

ISSN

0165-0270

Publication Date

August 1996

Volume

67

Issue

2

Start / End Page

221 / 231

Location

Netherlands

Related Subject Headings

  • Ultraviolet Rays
  • Spectrophotometry, Ultraviolet
  • Rats, Sprague-Dawley
  • Rats
  • Prefrontal Cortex
  • Photolysis
  • Neurology & Neurosurgery
  • Microelectrodes
  • Male
  • Magnetic Resonance Spectroscopy
 

Citation

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MLA
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Lee, T. H., Gee, K. R., Ellinwood, E. H., & Seidler, F. J. (1996). Combining 'caged-dopamine' photolysis with fast-scan cyclic voltammetry to assess dopamine clearance and release autoinhibition in vitro. J Neurosci Methods, 67(2), 221–231.
Lee, T. H., K. R. Gee, E. H. Ellinwood, and F. J. Seidler. “Combining 'caged-dopamine' photolysis with fast-scan cyclic voltammetry to assess dopamine clearance and release autoinhibition in vitro.J Neurosci Methods 67, no. 2 (August 1996): 221–31.
Journal cover image

Published In

J Neurosci Methods

ISSN

0165-0270

Publication Date

August 1996

Volume

67

Issue

2

Start / End Page

221 / 231

Location

Netherlands

Related Subject Headings

  • Ultraviolet Rays
  • Spectrophotometry, Ultraviolet
  • Rats, Sprague-Dawley
  • Rats
  • Prefrontal Cortex
  • Photolysis
  • Neurology & Neurosurgery
  • Microelectrodes
  • Male
  • Magnetic Resonance Spectroscopy