Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.
Publication
, Journal Article
Mukherjee, C; Lefkowitz, RJ
Published in: Proc Natl Acad Sci U S A
May 1976
Incubation of purified frog erythrocyte membranes with beta-adrenergic agonists at 25 degrees produces relatively rapid (half-time about 10 min) desensitization (inactivation) of about 60% of the beta-adrenergic receptor binding sites. The desensitized receptors no longer bind the specific beta-adrenergic ligand (-)[3H]dihydroalprenolol. The decrease in the number of functional beta-adrenergic receptors is also manifest as a decreased ability of isoproterenol to stimulate the membrane-bound adenylate cyclase.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
May 1976
Volume
73
Issue
5
Start / End Page
1494 / 1498
Location
United States
Related Subject Headings
- Structure-Activity Relationship
- Receptors, Adrenergic
- Purine Nucleotides
- Propranolol
- Kinetics
- Isoproterenol
- Guanosine Triphosphate
- Erythrocytes
- Enzyme Activation
- Dose-Response Relationship, Drug
Citation
APA
Chicago
ICMJE
MLA
NLM
Mukherjee, C., & Lefkowitz, R. J. (1976). Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides. Proc Natl Acad Sci U S A, 73(5), 1494–1498. https://doi.org/10.1073/pnas.73.5.1494
Mukherjee, C., and R. J. Lefkowitz. “Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.” Proc Natl Acad Sci U S A 73, no. 5 (May 1976): 1494–98. https://doi.org/10.1073/pnas.73.5.1494.
Mukherjee C, Lefkowitz RJ. Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides. Proc Natl Acad Sci U S A. 1976 May;73(5):1494–8.
Mukherjee, C., and R. J. Lefkowitz. “Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.” Proc Natl Acad Sci U S A, vol. 73, no. 5, May 1976, pp. 1494–98. Pubmed, doi:10.1073/pnas.73.5.1494.
Mukherjee C, Lefkowitz RJ. Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides. Proc Natl Acad Sci U S A. 1976 May;73(5):1494–1498.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
May 1976
Volume
73
Issue
5
Start / End Page
1494 / 1498
Location
United States
Related Subject Headings
- Structure-Activity Relationship
- Receptors, Adrenergic
- Purine Nucleotides
- Propranolol
- Kinetics
- Isoproterenol
- Guanosine Triphosphate
- Erythrocytes
- Enzyme Activation
- Dose-Response Relationship, Drug