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Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells.

Publication ,  Journal Article
Cao, B; Jia, J; Ma, L; Di, L; Song, G; Yuan, Y; Ma, B; Zhu, Y; Yu, J; Wang, X; Zhou, X; Lyerly, HK; Ren, J
Published in: Clin Transl Oncol
August 2012

PURPOSE: To ascertain the biologic significance of lung cancer Side population (SP) cells, which represent putative cancer stem cells (CSC) in the absence of consensus biomarkers for tumor-specific CSC. MATERIALS AND METHODS: We sorted and analyzed the angiogenic features of SP cells, isolated from tumor cell lines based on the exclusion of the DNA dye Hoechst 33342, from the NSCLC cell lines A549 and H460. RESULTS: Compared with non-SP cells, mRNA of vascular endothelial growth factor (VEGF)-A, VEGF-B, angiopoietin (ang)-1, ang-2, fibroblast growth factor-2 (FGF-2), cyclooxygenase-2 (Cox-2) and interleukin-8 (IL-8) were over-expressed in SP cells accompanied by over-expression of ABCG2 and MDR1 mRNA. The supernatant of cultured SP cells could significantly induce migration of human umbilical vein endothelial cells, while recombinant human endostatin (Endostar 25(®)) could inhibit the migration. CONCLUSIONS: This study revealed that the NSCLC SP cells might represent CSCs and possess pro-angiogenic properties, and antiangiogenesis represent a potential therapy.

Duke Scholars

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Published In

Clin Transl Oncol

DOI

EISSN

1699-3055

Publication Date

August 2012

Volume

14

Issue

8

Start / End Page

575 / 585

Location

Italy

Related Subject Headings

  • Side-Population Cells
  • Recombinant Proteins
  • RNA, Messenger
  • Phenotype
  • Oncology & Carcinogenesis
  • Neovascularization, Pathologic
  • Neoplastic Stem Cells
  • Neoplasm Proteins
  • Lung Neoplasms
  • Humans
 

Citation

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Chicago
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Cao, B., Jia, J., Ma, L., Di, L., Song, G., Yuan, Y., … Ren, J. (2012). Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells. Clin Transl Oncol, 14(8), 575–585. https://doi.org/10.1007/s12094-012-0844-9
Cao, Baoshan, Jun Jia, Liwen Ma, Lijun Di, Guohong Song, Yanhua Yuan, Bo Ma, et al. “Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells.Clin Transl Oncol 14, no. 8 (August 2012): 575–85. https://doi.org/10.1007/s12094-012-0844-9.
Cao B, Jia J, Ma L, Di L, Song G, Yuan Y, et al. Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells. Clin Transl Oncol. 2012 Aug;14(8):575–85.
Cao, Baoshan, et al. “Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells.Clin Transl Oncol, vol. 14, no. 8, Aug. 2012, pp. 575–85. Pubmed, doi:10.1007/s12094-012-0844-9.
Cao B, Jia J, Ma L, Di L, Song G, Yuan Y, Ma B, Zhu Y, Yu J, Wang X, Zhou X, Lyerly HK, Ren J. Recombinant human endostatin could eliminate the pro-angiogenesis priority of SP cells sorted from non-small cell lung cancer cells. Clin Transl Oncol. 2012 Aug;14(8):575–585.
Journal cover image

Published In

Clin Transl Oncol

DOI

EISSN

1699-3055

Publication Date

August 2012

Volume

14

Issue

8

Start / End Page

575 / 585

Location

Italy

Related Subject Headings

  • Side-Population Cells
  • Recombinant Proteins
  • RNA, Messenger
  • Phenotype
  • Oncology & Carcinogenesis
  • Neovascularization, Pathologic
  • Neoplastic Stem Cells
  • Neoplasm Proteins
  • Lung Neoplasms
  • Humans