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Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats.

Publication ,  Journal Article
Tsukamoto, K; Ariga, H; Mantyh, C; Pappas, TN; Yanagi, H; Yamamura, T; Takahashi, T
Published in: Am J Physiol Regul Integr Comp Physiol
July 2007

Enterochromaffin (EC) cells of the epithelial cells release 5-HT into the lumen, as well as basolateral border. However, the physiological role of released 5-HT into the lumen is poorly understood. Concentrations of 5-HT in the colonic mucosa, colonic lumen, and feces were measured by HPLC in rats. To investigate whether intraluminal 5-HT accelerates colonic transit, 5-HT and (51)Cr were administered into the lumen of the proximal colon, and colonic transit was measured. To investigate whether 5-HT is released into the lumen, we used an ex vivo model of isolated vascularly and luminally perfused rat proximal colon. To investigate whether luminal 5-HT is involved in regulating stress-induced colonic motility, the distal colonic motility was recorded under the stress loading, and a 5-HT(3) receptor antagonist (ondansetron, 10(-6) M, 0.5 ml) was administered intraluminally of the distal colon. Tissue content of 5-HT in the proximal colon (15.2 +/- 4.3 ng/mg wet tissue) was significantly higher than that in the distal colon (3.3 +/- 0.7 ng/mg wet tissue), while fecal content and luminal concentration of 5-HT was almost the same between the proximal and distal colon. Luminal administration of 5-HT (10(-6)-10(-5) M) significantly accelerated colonic transit. Elevation of intraluminal pressure by 10 cmH(2)O significantly increased the luminal concentration of 5-HT but not the vascular concentration of 5-HT. Stress-induced stimulation of the distal colonic motility was significantly attenuated by the luminal administration of ondansetron. These results suggest that luminally released 5-HT from EC cells plays an important role in regulating colonic motility in rats.

Duke Scholars

Published In

Am J Physiol Regul Integr Comp Physiol

DOI

ISSN

0363-6119

Publication Date

July 2007

Volume

293

Issue

1

Start / End Page

R64 / R69

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Tetrodotoxin
  • Stress, Psychological
  • Serotonin Antagonists
  • Serotonin
  • Restraint, Physical
  • Rats, Sprague-Dawley
  • Rats
  • Physiology
  • Ondansetron
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tsukamoto, K., Ariga, H., Mantyh, C., Pappas, T. N., Yanagi, H., Yamamura, T., & Takahashi, T. (2007). Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats. Am J Physiol Regul Integr Comp Physiol, 293(1), R64–R69. https://doi.org/10.1152/ajpregu.00856.2006
Tsukamoto, Kiyoshi, Hajime Ariga, Chris Mantyh, Theodore N. Pappas, Hidenori Yanagi, Takehira Yamamura, and Toku Takahashi. “Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats.Am J Physiol Regul Integr Comp Physiol 293, no. 1 (July 2007): R64–69. https://doi.org/10.1152/ajpregu.00856.2006.
Tsukamoto K, Ariga H, Mantyh C, Pappas TN, Yanagi H, Yamamura T, et al. Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats. Am J Physiol Regul Integr Comp Physiol. 2007 Jul;293(1):R64–9.
Tsukamoto, Kiyoshi, et al. “Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats.Am J Physiol Regul Integr Comp Physiol, vol. 293, no. 1, July 2007, pp. R64–69. Pubmed, doi:10.1152/ajpregu.00856.2006.
Tsukamoto K, Ariga H, Mantyh C, Pappas TN, Yanagi H, Yamamura T, Takahashi T. Luminally released serotonin stimulates colonic motility and accelerates colonic transit in rats. Am J Physiol Regul Integr Comp Physiol. 2007 Jul;293(1):R64–R69.

Published In

Am J Physiol Regul Integr Comp Physiol

DOI

ISSN

0363-6119

Publication Date

July 2007

Volume

293

Issue

1

Start / End Page

R64 / R69

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Tetrodotoxin
  • Stress, Psychological
  • Serotonin Antagonists
  • Serotonin
  • Restraint, Physical
  • Rats, Sprague-Dawley
  • Rats
  • Physiology
  • Ondansetron