A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.
Publication
, Journal Article
DasGupta, R; Nybakken, K; Booker, M; Mathey-Prevot, B; Gonsalves, F; Changkakoty, B; Perrimon, N
Published in: Genome biology
January 2007
Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
Duke Scholars
Published In
Genome biology
DOI
EISSN
1474-760X
ISSN
1474-7596
Publication Date
January 2007
Volume
8
Issue
9
Start / End Page
R203
Related Subject Headings
- Transcription, Genetic
- Sensitivity and Specificity
- RNA, Double-Stranded
- RNA Interference
- RNA
- Models, Genetic
- Genome
- Genetic Techniques
- Genes, Insect
- Gene Library
Citation
APA
Chicago
ICMJE
MLA
NLM
DasGupta, R., Nybakken, K., Booker, M., Mathey-Prevot, B., Gonsalves, F., Changkakoty, B., & Perrimon, N. (2007). A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome Biology, 8(9), R203. https://doi.org/10.1186/gb-2007-8-9-r203
DasGupta, Ramanuj, Kent Nybakken, Matthew Booker, Bernard Mathey-Prevot, Foster Gonsalves, Binita Changkakoty, and Norbert Perrimon. “A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.” Genome Biology 8, no. 9 (January 2007): R203. https://doi.org/10.1186/gb-2007-8-9-r203.
DasGupta R, Nybakken K, Booker M, Mathey-Prevot B, Gonsalves F, Changkakoty B, et al. A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome biology. 2007 Jan;8(9):R203.
DasGupta, Ramanuj, et al. “A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.” Genome Biology, vol. 8, no. 9, Jan. 2007, p. R203. Epmc, doi:10.1186/gb-2007-8-9-r203.
DasGupta R, Nybakken K, Booker M, Mathey-Prevot B, Gonsalves F, Changkakoty B, Perrimon N. A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome biology. 2007 Jan;8(9):R203.
Published In
Genome biology
DOI
EISSN
1474-760X
ISSN
1474-7596
Publication Date
January 2007
Volume
8
Issue
9
Start / End Page
R203
Related Subject Headings
- Transcription, Genetic
- Sensitivity and Specificity
- RNA, Double-Stranded
- RNA Interference
- RNA
- Models, Genetic
- Genome
- Genetic Techniques
- Genes, Insect
- Gene Library