Applications of high-throughput RNA interference screens to problems in cell and developmental biology.
Publication
, Journal Article
Perrimon, N; Mathey-Prevot, B
Published in: Genetics
January 2007
RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Genetics
DOI
ISSN
0016-6731
Publication Date
January 2007
Volume
175
Issue
1
Start / End Page
7 / 16
Location
United States
Related Subject Headings
- RNA Interference
- Phenotype
- Humans
- Genome
- Developmental Biology
- Developmental Biology
- Animals
- 3105 Genetics
- 3101 Biochemistry and cell biology
- 0604 Genetics
Citation
APA
Chicago
ICMJE
MLA
NLM
Perrimon, N., & Mathey-Prevot, B. (2007). Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics, 175(1), 7–16. https://doi.org/10.1534/genetics.106.069963
Perrimon, Norbert, and Bernard Mathey-Prevot. “Applications of high-throughput RNA interference screens to problems in cell and developmental biology.” Genetics 175, no. 1 (January 2007): 7–16. https://doi.org/10.1534/genetics.106.069963.
Perrimon N, Mathey-Prevot B. Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics. 2007 Jan;175(1):7–16.
Perrimon, Norbert, and Bernard Mathey-Prevot. “Applications of high-throughput RNA interference screens to problems in cell and developmental biology.” Genetics, vol. 175, no. 1, Jan. 2007, pp. 7–16. Pubmed, doi:10.1534/genetics.106.069963.
Perrimon N, Mathey-Prevot B. Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics. 2007 Jan;175(1):7–16.
Published In
Genetics
DOI
ISSN
0016-6731
Publication Date
January 2007
Volume
175
Issue
1
Start / End Page
7 / 16
Location
United States
Related Subject Headings
- RNA Interference
- Phenotype
- Humans
- Genome
- Developmental Biology
- Developmental Biology
- Animals
- 3105 Genetics
- 3101 Biochemistry and cell biology
- 0604 Genetics