Minimizing the risk of reporting false positives in large-scale RNAi screens.
Publication
, Journal Article
Echeverri, CJ; Beachy, PA; Baum, B; Boutros, M; Buchholz, F; Chanda, SK; Downward, J; Ellenberg, J; Fraser, AG; Hacohen, N; Hahn, WC; Kiger, A ...
Published in: Nat Methods
October 2006
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to validate results from these studies, if left unchecked, could eventually begin to undermine the credibility of RNAi as a powerful functional approach. This Commentary is an invitation to an open discussion started among various users of RNAi to set forth accepted standards that would insure the quality and accuracy of information in the large datasets coming out of genome-scale screens.
Duke Scholars
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Published In
Nat Methods
DOI
ISSN
1548-7091
Publication Date
October 2006
Volume
3
Issue
10
Start / End Page
777 / 779
Location
United States
Related Subject Headings
- Sensitivity and Specificity
- Risk
- RNA Interference
- Phenotype
- Genetic Testing
- False Positive Reactions
- Developmental Biology
- Databases, Genetic
- 31 Biological sciences
- 11 Medical and Health Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Echeverri, C. J., Beachy, P. A., Baum, B., Boutros, M., Buchholz, F., Chanda, S. K., … Bernards, R. (2006). Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods, 3(10), 777–779. https://doi.org/10.1038/nmeth1006-777
Echeverri, Christophe J., Philip A. Beachy, Buzz Baum, Michael Boutros, Frank Buchholz, Sumit K. Chanda, Julian Downward, et al. “Minimizing the risk of reporting false positives in large-scale RNAi screens.” Nat Methods 3, no. 10 (October 2006): 777–79. https://doi.org/10.1038/nmeth1006-777.
Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, et al. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods. 2006 Oct;3(10):777–9.
Echeverri, Christophe J., et al. “Minimizing the risk of reporting false positives in large-scale RNAi screens.” Nat Methods, vol. 3, no. 10, Oct. 2006, pp. 777–79. Pubmed, doi:10.1038/nmeth1006-777.
Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG, Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L, Ma Y, Mathey-Prévôt B, Root DE, Sabatini DM, Taipale J, Perrimon N, Bernards R. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods. 2006 Oct;3(10):777–779.
Published In
Nat Methods
DOI
ISSN
1548-7091
Publication Date
October 2006
Volume
3
Issue
10
Start / End Page
777 / 779
Location
United States
Related Subject Headings
- Sensitivity and Specificity
- Risk
- RNA Interference
- Phenotype
- Genetic Testing
- False Positive Reactions
- Developmental Biology
- Databases, Genetic
- 31 Biological sciences
- 11 Medical and Health Sciences