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Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.

Publication ,  Journal Article
Samarut, J; Mathey-Prevot, B; Hanafusa, H
Published in: Mol Cell Biol
May 1985

We have studied the expression of the protein kinase activity of NCP98, the c-fps gene product, in several hemopoietic tissues of chickens as a function of the developmental stage of these organs. We found that in bone marrow, spleen, and bursa, maximum NCP98 kinase activity on a per-cell basis correlates with the peak of granulopoiesis in these organs. Furthermore, in a bovine serum albumin density gradient fractionation of bone marrow cells, granulocytic cells appeared to account for most of the NCP98 kinase activity. No correlation was found between the distribution of erythrocytic, lymphocytic, or thrombocytic cells and the distribution of the expression of NCP98 kinase activity. However, NCP98 protein and kinase activity were 10-fold higher in macrophages than in bone marrow. In addition, depletion by complement-mediated lysis of erythrocytic cells in bone marrow did not significantly reduce the total recovery of NCP98 kinase activity. These results argue for the specific expression of the c-fps gene product in granulocytic cells and macrophages.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1985

Volume

5

Issue

5

Start / End Page

1067 / 1072

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Protein Kinases
  • Phosphoproteins
  • Oncogenes
  • Macrophages
  • Hematopoietic Stem Cells
  • Hematopoiesis
  • Granulocytes
 

Citation

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Samarut, J., Mathey-Prevot, B., & Hanafusa, H. (1985). Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells. Mol Cell Biol, 5(5), 1067–1072. https://doi.org/10.1128/mcb.5.5.1067-1072.1985
Samarut, J., B. Mathey-Prevot, and H. Hanafusa. “Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.Mol Cell Biol 5, no. 5 (May 1985): 1067–72. https://doi.org/10.1128/mcb.5.5.1067-1072.1985.
Samarut J, Mathey-Prevot B, Hanafusa H. Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells. Mol Cell Biol. 1985 May;5(5):1067–72.
Samarut, J., et al. “Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.Mol Cell Biol, vol. 5, no. 5, May 1985, pp. 1067–72. Pubmed, doi:10.1128/mcb.5.5.1067-1072.1985.
Samarut J, Mathey-Prevot B, Hanafusa H. Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells. Mol Cell Biol. 1985 May;5(5):1067–1072.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1985

Volume

5

Issue

5

Start / End Page

1067 / 1072

Location

United States

Related Subject Headings

  • Tissue Distribution
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Protein Kinases
  • Phosphoproteins
  • Oncogenes
  • Macrophages
  • Hematopoietic Stem Cells
  • Hematopoiesis
  • Granulocytes