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Synergism of accessory factors in functional expression of mammalian odorant receptors.

Publication ,  Journal Article
Zhuang, H; Matsunami, H
Published in: J Biol Chem
May 18, 2007

The discovery of odorant receptors led to endeavors in matching them with their cognate ligands. Although it has been challenging to functionally express odorant receptors in heterologous cells, previous studies have linked efficient odorant receptor expression with N-terminal modifications and accessory proteins, including the receptor-transporting proteins (RTPs) and Ric8b. Here we have shown that a shorter form of RTP1, RTP1S, supports robust cell-surface and functional expression of representative odorant receptors. Using a combination of accessory proteins, including RTP1S, Ric8b, and G(alphaolf), a diverse set of untagged odorant receptors were successfully expressed heterologously due to the synergistic effects among the various accessory proteins. Furthermore, the addition of an N-terminal rhodopsin tag to the odorant receptors, along with the same set of accessory proteins, exhibits an additional level of synergism, inducing enhanced odorant receptor responses to odorants and thus defining a more efficient heterologous expression system. We then showed that the presence or absence of different N-terminal tags has little effect on the ligand specificity of odorant receptors, although the amount of receptor expressed can play a role in the ligand response profile. The accuracy of the odorant receptor heterologous expression system involving tagged odorant receptors and various accessory proteins promises success in high throughput de-orphaning of mammalian odorant receptors.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 18, 2007

Volume

282

Issue

20

Start / End Page

15284 / 15293

Location

United States

Related Subject Headings

  • Recombinant Fusion Proteins
  • Receptors, Odorant
  • Nuclear Proteins
  • Membrane Transport Proteins
  • Humans
  • Gene Expression Regulation
  • Gene Expression
  • GTP-Binding Protein alpha Subunits
  • Cell Line
  • Biochemistry & Molecular Biology
 

Citation

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Zhuang, H., & Matsunami, H. (2007). Synergism of accessory factors in functional expression of mammalian odorant receptors. J Biol Chem, 282(20), 15284–15293. https://doi.org/10.1074/jbc.M700386200
Zhuang, Hanyi, and Hiroaki Matsunami. “Synergism of accessory factors in functional expression of mammalian odorant receptors.J Biol Chem 282, no. 20 (May 18, 2007): 15284–93. https://doi.org/10.1074/jbc.M700386200.
Zhuang H, Matsunami H. Synergism of accessory factors in functional expression of mammalian odorant receptors. J Biol Chem. 2007 May 18;282(20):15284–93.
Zhuang, Hanyi, and Hiroaki Matsunami. “Synergism of accessory factors in functional expression of mammalian odorant receptors.J Biol Chem, vol. 282, no. 20, May 2007, pp. 15284–93. Pubmed, doi:10.1074/jbc.M700386200.
Zhuang H, Matsunami H. Synergism of accessory factors in functional expression of mammalian odorant receptors. J Biol Chem. 2007 May 18;282(20):15284–15293.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 18, 2007

Volume

282

Issue

20

Start / End Page

15284 / 15293

Location

United States

Related Subject Headings

  • Recombinant Fusion Proteins
  • Receptors, Odorant
  • Nuclear Proteins
  • Membrane Transport Proteins
  • Humans
  • Gene Expression Regulation
  • Gene Expression
  • GTP-Binding Protein alpha Subunits
  • Cell Line
  • Biochemistry & Molecular Biology