DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae.
Dominant drug resistance markers offer experimental flexibility in the study of Saccharomyces cerevisiae by eliminating the dependence on auxotrophic mutations and, because they are phenotypically neutral, avoid the deleterious effects of auxotrophic mutations. We have developed a new dominant resistance marker, dsdAMX4, for use in the genetic manipulation of S. cerevisiae. The dsdA gene, which is derived from Escherichia coli and encodes a D-serine deaminase, confers to S. cerevisiae resistance to D-serine and the ability to use D-serine as a nitrogen source. Here we describe the construction of a dsdAMX4 cassette, capable of expression in S. cerevisiae, and the characterization of this new marker for use in chromosomal gene disruption. The unique selection properties of the dsdAMX4 cassette make it an important addition to the existing array of S. cerevisiae genetic tools.
Duke Scholars
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- Selection, Genetic
- Saccharomyces cerevisiae
- Mutagenesis, Insertional
- Hydro-Lyases
- Genetic Markers
- Escherichia coli
- 3107 Microbiology
- 1003 Industrial Biotechnology
- 0699 Other Biological Sciences
- 0605 Microbiology
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Selection, Genetic
- Saccharomyces cerevisiae
- Mutagenesis, Insertional
- Hydro-Lyases
- Genetic Markers
- Escherichia coli
- 3107 Microbiology
- 1003 Industrial Biotechnology
- 0699 Other Biological Sciences
- 0605 Microbiology