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Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.

Publication ,  Journal Article
Ito-Harashima, S; McCusker, JH
Published in: Yeast
January 15, 2004

Novel MX cassettes are described that contain the open reading frames (ORFs) of Saccharomyces cerevisiae or Candida albicans LYS5. The LYS5MX and CaLYS5MX cassettes, the targeting efficiencies of which are equivalent to those of other MX cassettes, are positively selected for Lys+ in a lys5 background. Unlike most of the other MX cassettes, the LYS5MX cassettes are also negatively selectable (alpha-aminoadipate-resistant), which will allow the use of the LYS5MX cassettes in plasmid shuffling and will also greatly facilitate marker recycling.

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Published In

Yeast

DOI

ISSN

0749-503X

Publication Date

January 15, 2004

Volume

21

Issue

1

Start / End Page

53 / 61

Location

England

Related Subject Headings

  • Transferases (Other Substituted Phosphate Groups)
  • Selection, Genetic
  • Saccharomyces cerevisiae
  • Polymerase Chain Reaction
  • Plasmids
  • Open Reading Frames
  • Mutagenesis, Insertional
  • Genetic Markers
  • Fungal Proteins
  • DNA, Fungal
 

Citation

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Ito-Harashima, S., & McCusker, J. H. (2004). Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. Yeast, 21(1), 53–61. https://doi.org/10.1002/yea.1057
Ito-Harashima, Sayoko, and John H. McCusker. “Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.Yeast 21, no. 1 (January 15, 2004): 53–61. https://doi.org/10.1002/yea.1057.
Ito-Harashima, Sayoko, and John H. McCusker. “Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.Yeast, vol. 21, no. 1, Jan. 2004, pp. 53–61. Pubmed, doi:10.1002/yea.1057.
Journal cover image

Published In

Yeast

DOI

ISSN

0749-503X

Publication Date

January 15, 2004

Volume

21

Issue

1

Start / End Page

53 / 61

Location

England

Related Subject Headings

  • Transferases (Other Substituted Phosphate Groups)
  • Selection, Genetic
  • Saccharomyces cerevisiae
  • Polymerase Chain Reaction
  • Plasmids
  • Open Reading Frames
  • Mutagenesis, Insertional
  • Genetic Markers
  • Fungal Proteins
  • DNA, Fungal