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Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes.

Publication ,  Journal Article
Pham, TA; McDonnell, DP; Tsai, MJ; O'Malley, BW
Published in: Biochemistry
February 11, 1992

In cells, steroid hormone receptors interact with target enhancer elements on nucleosomes to regulate transcription of genes. To elucidate how nucleosomes can potentially regulate the interactions of steroid receptors with steroid response elements, we have examined the effects of nucleosome positioning and histone source on the binding of the progesterone receptor to DNA elements on nucleosomes reconstituted in vitro. We find that the affinity of the receptor for its response element is dependent on the position of the element within the nucleosome, but not on the histone source, active or inactive chromatin. Our results suggest that the strength of DNA-histone interactions within the nucleosome modulates the binding of progesterone receptor to response elements. Thus, nucleosome positioning is likely to influence the function of steroid receptors in vivo.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

February 11, 1992

Volume

31

Issue

5

Start / End Page

1570 / 1578

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Protein Binding
  • Progesterone
  • Nucleosomes
  • Molecular Sequence Data
  • Histones
  • Erythrocytes
  • Enhancer Elements, Genetic
  • DNA
  • Chickens
 

Citation

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Pham, T. A., McDonnell, D. P., Tsai, M. J., & O’Malley, B. W. (1992). Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes. Biochemistry, 31(5), 1570–1578. https://doi.org/10.1021/bi00120a039
Pham, T. A., D. P. McDonnell, M. J. Tsai, and B. W. O’Malley. “Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes.Biochemistry 31, no. 5 (February 11, 1992): 1570–78. https://doi.org/10.1021/bi00120a039.
Pham TA, McDonnell DP, Tsai MJ, O’Malley BW. Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes. Biochemistry. 1992 Feb 11;31(5):1570–8.
Pham, T. A., et al. “Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes.Biochemistry, vol. 31, no. 5, Feb. 1992, pp. 1570–78. Pubmed, doi:10.1021/bi00120a039.
Pham TA, McDonnell DP, Tsai MJ, O’Malley BW. Modulation of progesterone receptor binding to progesterone response elements by positioned nucleosomes. Biochemistry. 1992 Feb 11;31(5):1570–1578.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

February 11, 1992

Volume

31

Issue

5

Start / End Page

1570 / 1578

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Protein Binding
  • Progesterone
  • Nucleosomes
  • Molecular Sequence Data
  • Histones
  • Erythrocytes
  • Enhancer Elements, Genetic
  • DNA
  • Chickens