The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.
Publication
, Journal Article
Zhang, X-X; Prata, CAH; McIntosh, TJ; Barthélémy, P; Grinstaff, MW
Published in: Bioconjug Chem
May 19, 2010
A series of charge-reversal amphiphiles with different spacers separating the headgroup from the hydrophobic chains are described for delivery of DNA and siRNA. Among them, the amphiphiles possessing a glycine spacer (e.g., B-GlyGly) showed effective DNA transfection in CHO and NIH 3T3 cells, as well as siRNA gene knockdown in HepG2 and UASMC cells. Ethidium bromide quenching assays revealed that DNA was released the fastest from the lipoplex of B-GlyGly in the presence of esterase. Also, X-ray diffraction results indicated that the DNA was located between the adjacent lipid bilayers in the lipoplex of B-GlyGly. These distinct features appear to be required for high transfection activity.
Duke Scholars
Published In
Bioconjug Chem
DOI
EISSN
1520-4812
Publication Date
May 19, 2010
Volume
21
Issue
5
Start / End Page
988 / 993
Location
United States
Related Subject Headings
- Transfection
- Surface-Active Agents
- RNA, Small Interfering
- Organic Chemistry
- NIH 3T3 Cells
- Mice
- Humans
- Glycylglycine
- Gene Knockdown Techniques
- DNA
Citation
APA
Chicago
ICMJE
MLA
NLM
Zhang, X.-X., Prata, C. A. H., McIntosh, T. J., Barthélémy, P., & Grinstaff, M. W. (2010). The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery. Bioconjug Chem, 21(5), 988–993. https://doi.org/10.1021/bc9005464
Zhang, Xiao-Xiang, Carla A. H. Prata, Thomas J. McIntosh, Philippe Barthélémy, and Mark W. Grinstaff. “The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.” Bioconjug Chem 21, no. 5 (May 19, 2010): 988–93. https://doi.org/10.1021/bc9005464.
Zhang X-X, Prata CAH, McIntosh TJ, Barthélémy P, Grinstaff MW. The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery. Bioconjug Chem. 2010 May 19;21(5):988–93.
Zhang, Xiao-Xiang, et al. “The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.” Bioconjug Chem, vol. 21, no. 5, May 2010, pp. 988–93. Pubmed, doi:10.1021/bc9005464.
Zhang X-X, Prata CAH, McIntosh TJ, Barthélémy P, Grinstaff MW. The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery. Bioconjug Chem. 2010 May 19;21(5):988–993.
Published In
Bioconjug Chem
DOI
EISSN
1520-4812
Publication Date
May 19, 2010
Volume
21
Issue
5
Start / End Page
988 / 993
Location
United States
Related Subject Headings
- Transfection
- Surface-Active Agents
- RNA, Small Interfering
- Organic Chemistry
- NIH 3T3 Cells
- Mice
- Humans
- Glycylglycine
- Gene Knockdown Techniques
- DNA