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Bilayer interfacial properties modulate the binding of amphipathic peptides.

Publication ,  Journal Article
Allende, D; Vidal, A; Simon, SA; McIntosh, TJ
Published in: Chem Phys Lipids
January 2003

The free energy of transfer (DeltaG degrees ) from water to lipid bilayers was measured for two amphipathic peptides, the presequence of the mitochondrial peptide rhodanese (MPR) and melittin. Experiments were designed to determine the effects on peptide partitioning of the addition of lipids that produce structural modifications to the bilayer/water interface. In particular, the addition of cholesterol or the cholesterol analog 6-ketocholestanol increases the bilayer area compressibility modulus, indicating that these molecules modify lipid-lipid interactions in the plane of the bilayer. The addition of 6-ketocholestanol or lipids with attached polyethylene glycol chains (PEG-lipids) modify the effective thickness of the interfacial region; 6-ketocholestanol increases the width of hydrophilic headgroup region in the direction of the acyl chains whereas the protruding PEG chains of PEG-lipids increase the structural width of the headgroup region into the surrounding aqueous phase. The incorporation of PEG-lipids with PEG molecular weights of 2000 or 5000 had no appreciable effect on peptide partitioning that could not be accounted for by the presence of surface charge. However, for both MPR and melittin DeltaG degrees decreased linearly with increasing bilayer compressibility modulus, demonstrating the importance of bilayer mechanical properties in the binding of amphipathic peptides.

Duke Scholars

Published In

Chem Phys Lipids

DOI

ISSN

0009-3084

Publication Date

January 2003

Volume

122

Issue

1-2

Start / End Page

65 / 76

Location

Ireland

Related Subject Headings

  • X-Ray Diffraction
  • Protein Binding
  • Peptides
  • Melitten
  • Liposomes
  • Lipid Bilayers
  • Cholesterol
  • Biophysics
  • 3205 Medical biochemistry and metabolomics
  • 3101 Biochemistry and cell biology
 

Citation

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Allende, D., Vidal, A., Simon, S. A., & McIntosh, T. J. (2003). Bilayer interfacial properties modulate the binding of amphipathic peptides. Chem Phys Lipids, 122(1–2), 65–76. https://doi.org/10.1016/s0009-3084(02)00179-2
Allende, Daniel, Adriana Vidal, Sidney A. Simon, and Thomas J. McIntosh. “Bilayer interfacial properties modulate the binding of amphipathic peptides.Chem Phys Lipids 122, no. 1–2 (January 2003): 65–76. https://doi.org/10.1016/s0009-3084(02)00179-2.
Allende D, Vidal A, Simon SA, McIntosh TJ. Bilayer interfacial properties modulate the binding of amphipathic peptides. Chem Phys Lipids. 2003 Jan;122(1–2):65–76.
Allende, Daniel, et al. “Bilayer interfacial properties modulate the binding of amphipathic peptides.Chem Phys Lipids, vol. 122, no. 1–2, Jan. 2003, pp. 65–76. Pubmed, doi:10.1016/s0009-3084(02)00179-2.
Allende D, Vidal A, Simon SA, McIntosh TJ. Bilayer interfacial properties modulate the binding of amphipathic peptides. Chem Phys Lipids. 2003 Jan;122(1–2):65–76.
Journal cover image

Published In

Chem Phys Lipids

DOI

ISSN

0009-3084

Publication Date

January 2003

Volume

122

Issue

1-2

Start / End Page

65 / 76

Location

Ireland

Related Subject Headings

  • X-Ray Diffraction
  • Protein Binding
  • Peptides
  • Melitten
  • Liposomes
  • Lipid Bilayers
  • Cholesterol
  • Biophysics
  • 3205 Medical biochemistry and metabolomics
  • 3101 Biochemistry and cell biology