Skip to main content
Journal cover image

Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals.

Publication ,  Journal Article
Kraus, JE; McNamara, JO
Published in: Brain Res Mol Brain Res
October 30, 1998

Kindling refers to a phenomenon in which repeated application of initially subconvulsive electrical stimulations produces limbic and clonic motor seizures of progressively increasing severity. Once established, the increased excitability is lifelong. A diversity of studies demonstrate that kindling results in long lasting (28 days) alterations of the functional and pharmacologic properties of NMDA receptors, indicating that kindling may cause changes intrinsic to the NMDA receptor itself. Our previous studies disclosed no differences in NMDA receptor subunit gene or splice isoform mRNA expression between control and kindled animals 28 days after the last kindled seizure. Here, we extend those earlier studies by measuring levels of subunit protein for NMDAR1, NR2A, and NR2B in the hippocampus of control and kindled animals, 28 days after the last kindled seizure. We report that kindling does not effect long-lasting changes in the levels of NMDA receptor subunit protein. Together these findings support the idea that alterations in NMDA receptor protein expression do not contribute to the novel properties of NMDA receptors induced by kindling.

Duke Scholars

Published In

Brain Res Mol Brain Res

DOI

ISSN

0169-328X

Publication Date

October 30, 1998

Volume

61

Issue

1-2

Start / End Page

114 / 120

Location

Netherlands

Related Subject Headings

  • Receptors, N-Methyl-D-Aspartate
  • Rats, Sprague-Dawley
  • Rats
  • Protein Isoforms
  • Neurology & Neurosurgery
  • Male
  • Kindling, Neurologic
  • Immunoblotting
  • Hippocampus
  • Antibody Specificity
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kraus, J. E., & McNamara, J. O. (1998). Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals. Brain Res Mol Brain Res, 61(1–2), 114–120. https://doi.org/10.1016/s0169-328x(98)00220-4
Kraus, J. E., and J. O. McNamara. “Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals.Brain Res Mol Brain Res 61, no. 1–2 (October 30, 1998): 114–20. https://doi.org/10.1016/s0169-328x(98)00220-4.
Kraus JE, McNamara JO. Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals. Brain Res Mol Brain Res. 1998 Oct 30;61(1–2):114–20.
Kraus, J. E., and J. O. McNamara. “Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals.Brain Res Mol Brain Res, vol. 61, no. 1–2, Oct. 1998, pp. 114–20. Pubmed, doi:10.1016/s0169-328x(98)00220-4.
Kraus JE, McNamara JO. Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals. Brain Res Mol Brain Res. 1998 Oct 30;61(1–2):114–120.
Journal cover image

Published In

Brain Res Mol Brain Res

DOI

ISSN

0169-328X

Publication Date

October 30, 1998

Volume

61

Issue

1-2

Start / End Page

114 / 120

Location

Netherlands

Related Subject Headings

  • Receptors, N-Methyl-D-Aspartate
  • Rats, Sprague-Dawley
  • Rats
  • Protein Isoforms
  • Neurology & Neurosurgery
  • Male
  • Kindling, Neurologic
  • Immunoblotting
  • Hippocampus
  • Antibody Specificity