Capillary Supercritical Fluid Chromatography—Mass Spectrometry Using A “High Mass” Quadrupole and Splitless Injection

Most capillary supercritical fluid chromatography-mass spectrometry (SFC-MS) has been done on instruments of mass range of less than 1500 Da, though SFC Is able to elute species of molecular masses in the thousands of daltons. In addition, splitting Injection is usually employed In SFC, which hampers quantitation. This work addresses both these problems by using a 3000-Da mass range quadrupole In combination with splltless Injection. A solution of poly(dl-methylslloxane) In carbon dioxide is introduced to the ion source by supercritical fluid injection for tuning purposes. Poly(dlmethylslloxanes) and derivatized oligosaccharides are transported Intact through the SFC-MS Interface. Simple ammonia chemical Ionization spectra, dominated by the ammonium adduct Ion, are seen to the mass limit of the Instrument. A signal-to-noise ratio of approximately 4 is measured for the ammonium adduct ion of 0.8 ng of methyl arachldate. © 1988, American Chemical Society. All rights reserved.

Duke Scholars

Author David Stuart Millington Pediatrics, Medical Genetics