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Identifying sequence similarities between DNA molecules.

Publication ,  Journal Article
Hoyt, PR; Doktycz, MJ; Modrich, P; Warmack, RJ; Allison, DP
Published in: Ultramicroscopy
February 2000

An atomic force microscope (AFM) imaging technique is described to compare sequences between two different DNA molecules and precisely locate nonhomologies in DNA strands. Sequence comparisons are made by forming heteroduplexes between the two molecules and, by AFM imaging the intact molecules formed, identifying both homologous and nonhomologous regions. By forming heteroduplexes between linearized wildtype pSV-beta-galactosidase plasmid (6821 bp) and a series of deletion mutants we have identified nonhomologies (deletions) as small as 22 bp and as large as 418 bp. Furthermore, by incorporating our technique for AFM-mediated restriction mapping of DNA these mutations can be positioned relative to EcoRI restriction sites. These results suggest AFM can be useful in identifying molecular level similarities and differences in DNA.

Duke Scholars

Published In

Ultramicroscopy

DOI

ISSN

0304-3991

Publication Date

February 2000

Volume

82

Issue

1-4

Start / End Page

237 / 244

Location

Netherlands

Related Subject Headings

  • Sequence Homology
  • Sequence Deletion
  • Plasmids
  • Nucleic Acid Heteroduplexes
  • Mutagenesis, Insertional
  • Microscopy, Atomic Force
  • Microscopy
  • Deoxyribonuclease EcoRI
  • DNA
  • Cloning, Molecular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hoyt, P. R., Doktycz, M. J., Modrich, P., Warmack, R. J., & Allison, D. P. (2000). Identifying sequence similarities between DNA molecules. Ultramicroscopy, 82(1–4), 237–244. https://doi.org/10.1016/s0304-3991(99)00153-9
Hoyt, P. R., M. J. Doktycz, P. Modrich, R. J. Warmack, and D. P. Allison. “Identifying sequence similarities between DNA molecules.Ultramicroscopy 82, no. 1–4 (February 2000): 237–44. https://doi.org/10.1016/s0304-3991(99)00153-9.
Hoyt PR, Doktycz MJ, Modrich P, Warmack RJ, Allison DP. Identifying sequence similarities between DNA molecules. Ultramicroscopy. 2000 Feb;82(1–4):237–44.
Hoyt, P. R., et al. “Identifying sequence similarities between DNA molecules.Ultramicroscopy, vol. 82, no. 1–4, Feb. 2000, pp. 237–44. Pubmed, doi:10.1016/s0304-3991(99)00153-9.
Hoyt PR, Doktycz MJ, Modrich P, Warmack RJ, Allison DP. Identifying sequence similarities between DNA molecules. Ultramicroscopy. 2000 Feb;82(1–4):237–244.
Journal cover image

Published In

Ultramicroscopy

DOI

ISSN

0304-3991

Publication Date

February 2000

Volume

82

Issue

1-4

Start / End Page

237 / 244

Location

Netherlands

Related Subject Headings

  • Sequence Homology
  • Sequence Deletion
  • Plasmids
  • Nucleic Acid Heteroduplexes
  • Mutagenesis, Insertional
  • Microscopy, Atomic Force
  • Microscopy
  • Deoxyribonuclease EcoRI
  • DNA
  • Cloning, Molecular