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Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs.

Publication ,  Journal Article
Worth, L; Clark, S; Radman, M; Modrich, P
Published in: Proc Natl Acad Sci U S A
April 12, 1994

Bacterial mutS and mutL mutations confer large increases in recombination between sequences that are divergent by several percent at the nucleotide level, an effect attributed to a role for products of these genes in control of recombination fidelity. Since MutS and MutL are proteins involved in the earliest steps of mismatch repair, including mismatch recognition by MutS, we have tested the possibility that they may affect strand exchange in response to occurrence of mispairs within the recombination heteroduplex. We show that MutS abolishes RecA-catalyzed strand transfer between fd and M13 bacteriophage DNAs, which vary by 3% at the nucleotide level, but is without effect on M13-M13 or fd-fd exchange. Although MutL alone has no effect on M13-fd heteroduplex formation, the protein dramatically enhances the inhibition of strand transfer mediated by MutS. Analysis of strand-transfer intermediates that accumulate in the presence of MutS and MutL indicates that the proteins block branch migration, presumably in response to occurrence of mispairs within newly formed heteroduplex.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 12, 1994

Volume

91

Issue

8

Start / End Page

3238 / 3241

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Rec A Recombinases
  • MutS DNA Mismatch-Binding Protein
  • MutL Proteins
  • Hydrogen Bonding
  • Escherichia coli Proteins
  • DNA-Binding Proteins
  • DNA, Viral
  • DNA, Single-Stranded
  • DNA Repair
 

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Worth, L., Clark, S., Radman, M., & Modrich, P. (1994). Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs. Proc Natl Acad Sci U S A, 91(8), 3238–3241. https://doi.org/10.1073/pnas.91.8.3238
Worth, L., S. Clark, M. Radman, and P. Modrich. “Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs.Proc Natl Acad Sci U S A 91, no. 8 (April 12, 1994): 3238–41. https://doi.org/10.1073/pnas.91.8.3238.
Worth L, Clark S, Radman M, Modrich P. Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3238–41.
Worth, L., et al. “Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs.Proc Natl Acad Sci U S A, vol. 91, no. 8, Apr. 1994, pp. 3238–41. Pubmed, doi:10.1073/pnas.91.8.3238.
Worth L, Clark S, Radman M, Modrich P. Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3238–3241.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 12, 1994

Volume

91

Issue

8

Start / End Page

3238 / 3241

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Rec A Recombinases
  • MutS DNA Mismatch-Binding Protein
  • MutL Proteins
  • Hydrogen Bonding
  • Escherichia coli Proteins
  • DNA-Binding Proteins
  • DNA, Viral
  • DNA, Single-Stranded
  • DNA Repair