Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.
Publication
, Journal Article
Modrich, P; Lehman, IR
Published in: Proc Natl Acad Sci U S A
May 1971
A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which is thermolabile. The formation of the ligase-adenylate intermediate is also temperature-sensitive in vitro. Two temperature-resistant revertants of the mutant contain normal amounts of a thermostable ligase. The mutant is killed by growth at 42 degrees C, a temperature at which it displays aberrant DNA synthesis. These results suggest that the ligase is necessary for normal DNA metabolism and viability in this strain.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
May 1971
Volume
68
Issue
5
Start / End Page
1002 / 1005
Location
United States
Related Subject Headings
- Tritium
- Thymidine
- Mutation
- Ligases
- Hot Temperature
- Genetics, Microbial
- Escherichia coli
- Edetic Acid
- DNA, Bacterial
- Adenine Nucleotides
Citation
APA
Chicago
ICMJE
MLA
NLM
Modrich, P., & Lehman, I. R. (1971). Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase. Proc Natl Acad Sci U S A, 68(5), 1002–1005. https://doi.org/10.1073/pnas.68.5.1002
Modrich, P., and I. R. Lehman. “Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.” Proc Natl Acad Sci U S A 68, no. 5 (May 1971): 1002–5. https://doi.org/10.1073/pnas.68.5.1002.
Modrich P, Lehman IR. Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase. Proc Natl Acad Sci U S A. 1971 May;68(5):1002–5.
Modrich, P., and I. R. Lehman. “Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.” Proc Natl Acad Sci U S A, vol. 68, no. 5, May 1971, pp. 1002–05. Pubmed, doi:10.1073/pnas.68.5.1002.
Modrich P, Lehman IR. Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase. Proc Natl Acad Sci U S A. 1971 May;68(5):1002–1005.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
May 1971
Volume
68
Issue
5
Start / End Page
1002 / 1005
Location
United States
Related Subject Headings
- Tritium
- Thymidine
- Mutation
- Ligases
- Hot Temperature
- Genetics, Microbial
- Escherichia coli
- Edetic Acid
- DNA, Bacterial
- Adenine Nucleotides