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Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein.

Publication ,  Journal Article
Dey, AK; Burke, B; Sun, Y; Hartog, K; Heeney, JL; Montefiori, D; Srivastava, IK; Barnett, SW
Published in: Vaccine
April 5, 2012

Identification of optimal antigen(s) and adjuvant combination(s) to elicit potent, protective, and long-lasting immunity has been a major challenge for the development of effective vaccines against chronic viral pathogens, such as HIV-1, for which there are not yet any licensed vaccines. Here we describe the use of a novel adjuvant approach employing Carbopol 971P(®) NF (hereafter referred to as Carbopol971P), a cross-linked polyanionic carbomer, in combination with the Novartis proprietary oil-in-water adjuvant, MF59, as a potentially safe and effective adjuvant to augment humoral immune responses to the HIV-1 envelope glycoprotein (Env). Intramuscular immunization of small animals with recombinant Env glycoprotein (gp140) formulated in Carbopol971P plus MF59 gave significantly higher titers of binding and virus neutralizing antibodies as compared to immunization using gp140 with either MF59 or Carbopol971P alone. In addition, the antibodies generated were of higher avidity. Importantly, the use of Carbopol971P plus MF59 did not cause any serious adverse reactions or any obvious health problems in animals upon intramuscular administration. Hence, the Carbopol971P plus MF59 adjuvant formulation may provide a benefit for future vaccine applications.

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Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

April 5, 2012

Volume

30

Issue

17

Start / End Page

2749 / 2759

Location

Netherlands

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Squalene
  • Rabbits
  • Protein Stability
  • Protein Binding
  • Polysorbates
  • Hydrogen-Ion Concentration
  • Humans
  • HIV-1
 

Citation

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Dey, A. K., Burke, B., Sun, Y., Hartog, K., Heeney, J. L., Montefiori, D., … Barnett, S. W. (2012). Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein. Vaccine, 30(17), 2749–2759. https://doi.org/10.1016/j.vaccine.2012.02.027
Dey, Antu K., Brian Burke, Yide Sun, Karin Hartog, Jonathan L. Heeney, David Montefiori, Indresh K. Srivastava, and Susan W. Barnett. “Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein.Vaccine 30, no. 17 (April 5, 2012): 2749–59. https://doi.org/10.1016/j.vaccine.2012.02.027.
Dey AK, Burke B, Sun Y, Hartog K, Heeney JL, Montefiori D, et al. Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein. Vaccine. 2012 Apr 5;30(17):2749–59.
Dey, Antu K., et al. “Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein.Vaccine, vol. 30, no. 17, Apr. 2012, pp. 2749–59. Pubmed, doi:10.1016/j.vaccine.2012.02.027.
Dey AK, Burke B, Sun Y, Hartog K, Heeney JL, Montefiori D, Srivastava IK, Barnett SW. Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein. Vaccine. 2012 Apr 5;30(17):2749–2759.
Journal cover image

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

April 5, 2012

Volume

30

Issue

17

Start / End Page

2749 / 2759

Location

Netherlands

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Squalene
  • Rabbits
  • Protein Stability
  • Protein Binding
  • Polysorbates
  • Hydrogen-Ion Concentration
  • Humans
  • HIV-1