Antibody-independent, complement-mediated enhancement of HIV-1 infection by mannosidase I and II inhibitors.
Human immunodeficiency virus type 1 (HIV-1) infectivity and cytopathic effect require proper maturation of the viral envelope glycoprotein carbohydrate moieties. We have found that fresh human serum enhances the infectivity of HIV-1 in MT-2 cell infection assays when virus is synthesized in the presence of the mannosidase I inhibitor, 1-deoxymannojirimycin, or the mannosidase II inhibitor, swainsonine, but has no enhancing effect on virus synthesized in the presence of the glucosidase I inhibitors, castanospermine and 1-deoxynojirimycin, or the glucosidase II inhibitor, bromoconduritol. Enhanced infections were characterized by cytopathic effect, antigen synthesis and reverse transcriptase release, all which occurred sooner than in control-infected cultures. This enhancement of infection was also observed in C1q-deficient serum but was not observed in serum that was heat-inactivated or depleted of complement components C3 or factor B, thus suggesting a requirement for the alternate pathway of complement.
Duke Scholars
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Related Subject Headings
- Virology
- Swainsonine
- RNA-Directed DNA Polymerase
- Mannosidases
- Humans
- HIV-1
- Glycosylation
- Glucosidases
- Glucosamine
- Fluorescent Antibody Technique
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Virology
- Swainsonine
- RNA-Directed DNA Polymerase
- Mannosidases
- Humans
- HIV-1
- Glycosylation
- Glucosidases
- Glucosamine
- Fluorescent Antibody Technique