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Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees.

Publication ,  Journal Article
Robinson, WE; Montefiori, DC; Mitchell, WM; Prince, AM; Alter, HJ; Dreesman, GR; Eichberg, JW
Published in: Proc Natl Acad Sci U S A
June 1989

Based on recent reports of antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected humans, sera from HIV-1 antibody-positive chimpanzees (Pan troglodytes) was evaluated for enhancing activity in an in vitro infection assay that uses MT-2 cells (a human lymphoblastoid cell line). Although fresh chimpanzee serum was found to have pronounced infection-enhancing properties in the absence of antibody to HIV-1, this effect was abolished by heat inactivation (57 degrees C, 1 hr) or treatment with cobra venom anticomplementary protein. Heat-inactivated, HIV-1 antibody-positive chimpanzee serum could enhance HIV-1 infection of MT-2 cells in vitro when combined with fresh, normal human serum. By serial serum samples from three HIV-1-infected chimpanzees, HIV-1 antibody-positive chimpanzees are shown to develop enhancing antibodies early in infection (2 mo postchallenge), whereas neutralizing antibodies develop later. Over the course of HIV-1 infection, this enhancing activity decreases while neutralizing activity increases, suggesting a possible role for enhancing and neutralizing activities in HIV-1 pathogenesis. The enhancing activity of an IgG fraction used to passively immunize chimpanzees against HIV-1 infection is shown to be present at dilutions as high as 1:65,000, offering an interesting possible reason for the failure of passive immunization to protect chimpanzees from HIV infection. These results suggest that serum from HIV-1-immunized chimpanzees might be tested to determine whether current HIV-1 candidate vaccines induce production of antibodies that mediate antibody-dependent enhancement of HIV-1 infection in this in vitro assay.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1989

Volume

86

Issue

12

Start / End Page

4710 / 4714

Location

United States

Related Subject Headings

  • RNA-Directed DNA Polymerase
  • Pan troglodytes
  • Immunization, Passive
  • HIV-1
  • Fluorescent Antibody Technique
  • Elapid Venoms
  • Cytotoxicity, Immunologic
  • Antibody-Dependent Cell Cytotoxicity
  • Animals
  • Acquired Immunodeficiency Syndrome
 

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Robinson, W. E., Montefiori, D. C., Mitchell, W. M., Prince, A. M., Alter, H. J., Dreesman, G. R., & Eichberg, J. W. (1989). Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees. Proc Natl Acad Sci U S A, 86(12), 4710–4714. https://doi.org/10.1073/pnas.86.12.4710
Robinson, W. E., D. C. Montefiori, W. M. Mitchell, A. M. Prince, H. J. Alter, G. R. Dreesman, and J. W. Eichberg. “Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees.Proc Natl Acad Sci U S A 86, no. 12 (June 1989): 4710–14. https://doi.org/10.1073/pnas.86.12.4710.
Robinson WE, Montefiori DC, Mitchell WM, Prince AM, Alter HJ, Dreesman GR, et al. Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4710–4.
Robinson, W. E., et al. “Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees.Proc Natl Acad Sci U S A, vol. 86, no. 12, June 1989, pp. 4710–14. Pubmed, doi:10.1073/pnas.86.12.4710.
Robinson WE, Montefiori DC, Mitchell WM, Prince AM, Alter HJ, Dreesman GR, Eichberg JW. Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected and passively immunized chimpanzees. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4710–4714.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1989

Volume

86

Issue

12

Start / End Page

4710 / 4714

Location

United States

Related Subject Headings

  • RNA-Directed DNA Polymerase
  • Pan troglodytes
  • Immunization, Passive
  • HIV-1
  • Fluorescent Antibody Technique
  • Elapid Venoms
  • Cytotoxicity, Immunologic
  • Antibody-Dependent Cell Cytotoxicity
  • Animals
  • Acquired Immunodeficiency Syndrome