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CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo.

Publication ,  Journal Article
Agadjanyan, MG; Kim, JJ; Trivedi, N; Wilson, DM; Monzavi-Karbassi, B; Morrison, LD; Nottingham, LK; Dentchev, T; Tsai, A; Dang, K; Chalian, AA ...
Published in: J Immunol
March 15, 1999

Activation of T cells requires both TCR-specific ligation by direct contact with peptide Ag-MHC complexes and coligation of the B7 family of ligands through CD28/CTLA-4 on the T cell surface. We recently reported that coadministration of CD86 cDNA along with DNA encoding HIV-1 Ags i.m. dramatically increased Ag-specific CTL responses. We investigated whether the bone marrow-derived professional APCs or muscle cells were responsible for the enhancement of CTL responses following CD86 coadministration. Accordingly, we analyzed CTL induction in bone marrow chimeras. These chimeras are capable of generating functional viral-specific CTLs against vaccinia virus and therefore represent a useful model system to study APC/T cell function in vivo. In vaccinated chimeras, we observed that only CD86 + Ag + MHC class I results in 1) detectable CTLs following in vitro restimulation, 2) detectable direct CTLs, 3) enhanced IFN-gamma production in an Ag-specific manner, and 4) dramatic tissue invasion of T cells. These results support that CD86 plays a central role in CTL induction in vivo, enabling non-bone marrow-derived cells to prime CTLs, a property previously associated solely with bone marrow-derived APCs.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 15, 1999

Volume

162

Issue

6

Start / End Page

3417 / 3427

Location

United States

Related Subject Headings

  • beta 2-Microglobulin
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Stem Cells
  • Radiation Chimera
  • Protein Engineering
  • Muscle, Skeletal
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
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MLA
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Agadjanyan, M. G., Kim, J. J., Trivedi, N., Wilson, D. M., Monzavi-Karbassi, B., Morrison, L. D., … Weiner, D. B. (1999). CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo. J Immunol, 162(6), 3417–3427.
Agadjanyan, M. G., J. J. Kim, N. Trivedi, D. M. Wilson, B. Monzavi-Karbassi, L. D. Morrison, L. K. Nottingham, et al. “CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo.J Immunol 162, no. 6 (March 15, 1999): 3417–27.
Agadjanyan MG, Kim JJ, Trivedi N, Wilson DM, Monzavi-Karbassi B, Morrison LD, et al. CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo. J Immunol. 1999 Mar 15;162(6):3417–27.
Agadjanyan, M. G., et al. “CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo.J Immunol, vol. 162, no. 6, Mar. 1999, pp. 3417–27.
Agadjanyan MG, Kim JJ, Trivedi N, Wilson DM, Monzavi-Karbassi B, Morrison LD, Nottingham LK, Dentchev T, Tsai A, Dang K, Chalian AA, Maldonado MA, Williams WV, Weiner DB. CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo. J Immunol. 1999 Mar 15;162(6):3417–3427.

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 15, 1999

Volume

162

Issue

6

Start / End Page

3417 / 3427

Location

United States

Related Subject Headings

  • beta 2-Microglobulin
  • Transfection
  • T-Lymphocytes, Cytotoxic
  • Stem Cells
  • Radiation Chimera
  • Protein Engineering
  • Muscle, Skeletal
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice