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Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo.

Publication ,  Journal Article
Kim, JJ; Tsai, A; Nottingham, LK; Morrison, L; Cunning, DM; Oh, J; Lee, DJ; Dang, K; Dentchev, T; Chalian, AA; Agadjanyan, MG; Weiner, DB
Published in: J Clin Invest
March 1999

The potential roles of adhesion molecules in the expansion of T cell-mediated immune responses in the periphery were examined using DNA immunogen constructs as model antigens. We coimmunized cDNA expression cassettes encoding the adhesion molecules intracellular adhesion molecule-1 (ICAM-1), lymphocyte function associated-3 (LFA-3), and vascular cell adhesion molecule-1 (VCAM-1) along with DNA immunogens, and we analyzed the resulting antigen-specific immune responses. We observed that antigen-specific T-cell responses can be enhanced by the coexpression of DNA immunogen and adhesion molecules ICAM-1 and LFA-3. Coexpression of ICAM-1 or LFA-3 molecules along with DNA immunogens resulted in a significant enhancement of T-helper cell proliferative responses. In addition, coimmunization with pCICAM-1 (and more moderately with pCLFA-3) resulted in a dramatic enhancement of CD8-restricted cytotoxic T-lymphocyte responses. Although VCAM-1 and ICAM-1 are similar in size, VCAM-1 coimmunization did not have any measurable effect on cell-mediated responses. These results suggest that ICAM-1 and LFA-3 provide direct T-cell costimulation. These observations are further supported by the finding that coinjection with ICAM-1 dramatically enhanced the level of interferon-gamma (IFN-gamma) and beta-chemokines macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and regulated on activation normal T-cell expression and secreted (RANTES) produced by stimulated T cells. Through comparative studies, we observed that ICAM-1/LFA-1 T-cell costimulatory pathways are independent of CD86/CD28 pathways and that they may synergistically expand T-cell responses in vivo.

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Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

March 1999

Volume

103

Issue

6

Start / End Page

869 / 877

Location

United States

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Vaccines, DNA
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes
  • Recombinant Proteins
  • Mice, Inbred BALB C
  • Mice
  • Membrane Glycoproteins
  • Macrophage Inflammatory Proteins
  • Lymphocyte Activation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kim, J. J., Tsai, A., Nottingham, L. K., Morrison, L., Cunning, D. M., Oh, J., … Weiner, D. B. (1999). Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo. J Clin Invest, 103(6), 869–877. https://doi.org/10.1172/JCI6024
Kim, J. J., A. Tsai, L. K. Nottingham, L. Morrison, D. M. Cunning, J. Oh, D. J. Lee, et al. “Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo.J Clin Invest 103, no. 6 (March 1999): 869–77. https://doi.org/10.1172/JCI6024.
Kim JJ, Tsai A, Nottingham LK, Morrison L, Cunning DM, Oh J, et al. Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo. J Clin Invest. 1999 Mar;103(6):869–77.
Kim, J. J., et al. “Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo.J Clin Invest, vol. 103, no. 6, Mar. 1999, pp. 869–77. Pubmed, doi:10.1172/JCI6024.
Kim JJ, Tsai A, Nottingham LK, Morrison L, Cunning DM, Oh J, Lee DJ, Dang K, Dentchev T, Chalian AA, Agadjanyan MG, Weiner DB. Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo. J Clin Invest. 1999 Mar;103(6):869–877.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

March 1999

Volume

103

Issue

6

Start / End Page

869 / 877

Location

United States

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Vaccines, DNA
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes
  • Recombinant Proteins
  • Mice, Inbred BALB C
  • Mice
  • Membrane Glycoproteins
  • Macrophage Inflammatory Proteins
  • Lymphocyte Activation