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Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype.

Publication ,  Journal Article
Kim, JJ; Maguire, HC; Nottingham, LK; Morrison, LD; Tsai, A; Sin, JI; Chalian, AA; Weiner, DB
Published in: J Interferon Cytokine Res
July 1998

Cytokines are important regulators of the immune response. They influence immune expression, the development of immunologic memory, and regulation of antigen-specific and nonspecific immune activation as well as allergic responses. In a model system in mice, we have studied the effect of plasmids expressing interleukin (IL)-10 or IL-12 on the modulation of antigen-specific responses. Coadministration of IL-12 or IL-10 genes with DNA immunogens directed the antigen-specific immune response toward a T helper (Th1)-type immunity. In addition to the modulation of antigen-specific immune responses, we studied the induction of delayed-type hypersensitivity (DTH) to contact allergens as an in vivo model of the Th1 response. We found that IL-12 and IL-10 gene-containing plasmids, and not the bacterial plasmid alone, upregulate this response. Our cytokine gene delivery technique demonstrates an important level of control of the magnitude and direction of induced immune responses and could be advantageous in a wide variety of immunotherapeutic strategies.

Duke Scholars

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Published In

J Interferon Cytokine Res

DOI

ISSN

1079-9907

Publication Date

July 1998

Volume

18

Issue

7

Start / End Page

537 / 547

Location

United States

Related Subject Headings

  • Up-Regulation
  • Th1 Cells
  • Plasmids
  • Mice, Inbred BALB C
  • Mice
  • Interleukin-12
  • Interleukin-10
  • Interferon-gamma
  • Immunophenotyping
  • Immunology
 

Citation

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Kim, J. J., Maguire, H. C., Nottingham, L. K., Morrison, L. D., Tsai, A., Sin, J. I., … Weiner, D. B. (1998). Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype. J Interferon Cytokine Res, 18(7), 537–547. https://doi.org/10.1089/jir.1998.18.537
Kim, J. J., H. C. Maguire, L. K. Nottingham, L. D. Morrison, A. Tsai, J. I. Sin, A. A. Chalian, and D. B. Weiner. “Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype.J Interferon Cytokine Res 18, no. 7 (July 1998): 537–47. https://doi.org/10.1089/jir.1998.18.537.
Kim JJ, Maguire HC, Nottingham LK, Morrison LD, Tsai A, Sin JI, et al. Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype. J Interferon Cytokine Res. 1998 Jul;18(7):537–47.
Kim, J. J., et al. “Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype.J Interferon Cytokine Res, vol. 18, no. 7, July 1998, pp. 537–47. Pubmed, doi:10.1089/jir.1998.18.537.
Kim JJ, Maguire HC, Nottingham LK, Morrison LD, Tsai A, Sin JI, Chalian AA, Weiner DB. Coadministration of IL-12 or IL-10 expression cassettes drives immune responses toward a Th1 phenotype. J Interferon Cytokine Res. 1998 Jul;18(7):537–547.
Journal cover image

Published In

J Interferon Cytokine Res

DOI

ISSN

1079-9907

Publication Date

July 1998

Volume

18

Issue

7

Start / End Page

537 / 547

Location

United States

Related Subject Headings

  • Up-Regulation
  • Th1 Cells
  • Plasmids
  • Mice, Inbred BALB C
  • Mice
  • Interleukin-12
  • Interleukin-10
  • Interferon-gamma
  • Immunophenotyping
  • Immunology