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CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines.

Publication ,  Journal Article
Kim, JJ; Nottingham, LK; Sin, JI; Tsai, A; Morrison, L; Oh, J; Dang, K; Hu, Y; Kazahaya, K; Bennett, M; Dentchev, T; Wilson, DM; Chalian, AA ...
Published in: J Clin Invest
September 15, 1998

The potential roles of CD8(+) T-cell-induced chemokines in the expansion of immune responses were examined using DNA immunogen constructs as model antigens. We coimmunized cDNA expression cassettes encoding the alpha-chemokines IL-8 and SDF-1alpha and the beta-chemokines MIP-1alpha, RANTES, and MCP-1 along with DNA immunogens and analyzed the resulting antigen-specific immune responses. In a manner more similar to the traditional immune modulatory role of CD4(+) T cells via the expression of Th1 or Th2 cytokines, CD8(+) T cells appeared to play an important role in immune expansion and effector function by producing chemokines. For instance, IL-8 was a strong inducer of CD4(+) T cells, indicated by strong T helper proliferative responses as well as an enhancement of antibody responses. MIP-1alpha had a dramatic effect on antibody responses and modulated the shift of immune responses to a Th2-type response. RANTES coimmunization enhanced the levels of antigen-specific Th1 and cytotoxic T lymphocyte (CTL) responses. Among the chemokines examined, MCP-1 was the most potent activator of CD8(+) CTL activity. The enhanced CTL results are supported by the increased expression of Th1 cytokines IFN-gamma and TNF-alpha and the reduction of IgG1/IgG2a ratio. Our results support that CD8(+) T cells may expand both humoral and cellular responses in vivo through the elaboration of specific chemokines at the peripheral site of infection during the effector stage of the immune response.

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Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

September 15, 1998

Volume

102

Issue

6

Start / End Page

1112 / 1124

Location

United States

Related Subject Headings

  • Vaccines, DNA
  • Th1 Cells
  • T-Lymphocytes, Cytotoxic
  • Models, Immunological
  • Mice, Inbred BALB C
  • Mice
  • Macrophage Inflammatory Proteins
  • Lymphocyte Activation
  • Interleukin-8
  • Immunology
 

Citation

APA
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MLA
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Kim, J. J., Nottingham, L. K., Sin, J. I., Tsai, A., Morrison, L., Oh, J., … Weiner, D. B. (1998). CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines. J Clin Invest, 102(6), 1112–1124. https://doi.org/10.1172/JCI3986
Kim, J. J., L. K. Nottingham, J. I. Sin, A. Tsai, L. Morrison, J. Oh, K. Dang, et al. “CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines.J Clin Invest 102, no. 6 (September 15, 1998): 1112–24. https://doi.org/10.1172/JCI3986.
Kim JJ, Nottingham LK, Sin JI, Tsai A, Morrison L, Oh J, et al. CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines. J Clin Invest. 1998 Sep 15;102(6):1112–24.
Kim, J. J., et al. “CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines.J Clin Invest, vol. 102, no. 6, Sept. 1998, pp. 1112–24. Pubmed, doi:10.1172/JCI3986.
Kim JJ, Nottingham LK, Sin JI, Tsai A, Morrison L, Oh J, Dang K, Hu Y, Kazahaya K, Bennett M, Dentchev T, Wilson DM, Chalian AA, Boyer JD, Agadjanyan MG, Weiner DB. CD8 positive T cells influence antigen-specific immune responses through the expression of chemokines. J Clin Invest. 1998 Sep 15;102(6):1112–1124.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

September 15, 1998

Volume

102

Issue

6

Start / End Page

1112 / 1124

Location

United States

Related Subject Headings

  • Vaccines, DNA
  • Th1 Cells
  • T-Lymphocytes, Cytotoxic
  • Models, Immunological
  • Mice, Inbred BALB C
  • Mice
  • Macrophage Inflammatory Proteins
  • Lymphocyte Activation
  • Interleukin-8
  • Immunology