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CD86 confers and directs MHC class I restricted CTL induction

Publication ,  Journal Article
Kim, JJ; Agadjanyan, MG; Trivedi, N; Wilson, DM; Morrison, LD; Maldonado, MA; Williams, WV; Weiner, DB
Published in: FASEB Journal
March 20, 1998

The complex process of CTL induction is only partially understood. Activation of T cells requires both TCR-specific ligation by direct contact with peptide antigen-MHC complexes and co-ligation of the B7 family of ligands with CD28/CTLA-4 on the T cell surface. We investigated the role of the CD80 and CD86 molecules in the amplification of in vivo immune responses. We developed expression cassettes for CD80 and CD86 and analyzed their ability to function as in vivo modulators of the immune response induced by DNA immunization cassettes against HFV-1 antigens by co-immunizing them into mice. We observed that co-immunization with CD86, not CD80 expression cassettes, resulted in a dramatic increase in cytotoxic T lymphocyte response which was both MHC class I-restricted and CD8+ T cell-dependent. Using bone marrow chimeric animals, we found that the MHC class I-restriction of CTL was determined to be under the providence of the CD86, even when this molecule and antigen were displayed on a non-professional antigen presenting cell background. This study defines a role of CD86 in CTL immune induction and raises, new questions about the presumed function of CD80 in the generation of antigen specific immunity.

Duke Scholars

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

March 20, 1998

Volume

12

Issue

5

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
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MLA
NLM
Kim, J. J., Agadjanyan, M. G., Trivedi, N., Wilson, D. M., Morrison, L. D., Maldonado, M. A., … Weiner, D. B. (1998). CD86 confers and directs MHC class I restricted CTL induction. FASEB Journal, 12(5).
Kim, J. J., M. G. Agadjanyan, N. Trivedi, D. M. Wilson, L. D. Morrison, M. A. Maldonado, W. V. Williams, and D. B. Weiner. “CD86 confers and directs MHC class I restricted CTL induction.” FASEB Journal 12, no. 5 (March 20, 1998).
Kim JJ, Agadjanyan MG, Trivedi N, Wilson DM, Morrison LD, Maldonado MA, et al. CD86 confers and directs MHC class I restricted CTL induction. FASEB Journal. 1998 Mar 20;12(5).
Kim, J. J., et al. “CD86 confers and directs MHC class I restricted CTL induction.” FASEB Journal, vol. 12, no. 5, Mar. 1998.
Kim JJ, Agadjanyan MG, Trivedi N, Wilson DM, Morrison LD, Maldonado MA, Williams WV, Weiner DB. CD86 confers and directs MHC class I restricted CTL induction. FASEB Journal. 1998 Mar 20;12(5).

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

March 20, 1998

Volume

12

Issue

5

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology