Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification
Publication
, Journal Article
Bodnar, WM; Bell, DJ; Blackburn, RK; Davis, RG; Flynn, HR; Forrest, RM; Krise, JM; Lee, MJ; Moseley, MA; Moyer, MB; Schlatzer, DM; Skehel, MJ
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
Automated in-gel digestion method coupled with MALDI/MS and MALDI/MS/MS for protein identification was analyzed. Three standard proteins at five different concentrations with four routinely used gel stains were used for the analysis. It was observed that an accurate assessment was obtained at a minimum level of 100 fmol loaded on gel. It was found the rms error for PMF using lock mass correction was 5 ppm or better and sequence coverage ranged from 6 to 28%. The results show that correct identification, of the three proteins analyzed, were obtained at value higher than or equals to 500 fmol loaded on gel.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
171 / 172
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Bodnar, W. M., Bell, D. J., Blackburn, R. K., Davis, R. G., Flynn, H. R., Forrest, R. M., … Skehel, M. J. (2002). Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 171–172.
Bodnar, W. M., D. J. Bell, R. K. Blackburn, R. G. Davis, H. R. Flynn, R. M. Forrest, J. M. Krise, et al. “Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 171–72.
Bodnar WM, Bell DJ, Blackburn RK, Davis RG, Flynn HR, Forrest RM, et al. Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;171–2.
Bodnar, W. M., et al. “Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 171–72.
Bodnar WM, Bell DJ, Blackburn RK, Davis RG, Flynn HR, Forrest RM, Krise JM, Lee MJ, Moseley MA, Moyer MB, Schlatzer DM, Skehel MJ. Evaluation of automated in-gel digestion coupled with MALDI/MS and MALDI/MS/MS for protein identification. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;171–172.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
171 / 172