Sensitive analysis of [D-Pen2,5]enkephalin in rat serum by capillary electrophoresis and laser-induced fluorescence detection.
A highly sensitive analytical method based on capillary zone electrophoresis (CZE) coupled with a laser-induced fluorescence (LIF) detector was explored for the analysis of [D-Pen2,5]enkephalin (DPDPE) in rat serum. DPDPE and the internal standard Phe-Leu-Glu-Glu-Ile (P9396) were extracted from serum samples with C18 solid-phase extraction disk cartridges, followed by derivatization with tetramethylrhodamine-5-isothiocyanate (TRITC) isomer G before introduction onto the capillary column. Complete resolution of DPDPE and the internal standard from other serum components was achieved within 20 min on a 140 cm x 50 microns I.D. capillary column with borate buffer (25 mM. pH 8.3). With the current method, it is possible to detect 1.3E-18 mol of DPDPE on column. The results suggest that CZE-LIF is a promising method for the sensitive and specific quantitation of therapeutic peptides in biological matrices.
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Related Subject Headings
- Spectrometry, Fluorescence
- Sensitivity and Specificity
- Rhodamines
- Receptors, Opioid, delta
- Rats
- Mass Spectrometry
- Lasers
- Hydrogen-Ion Concentration
- Fluorescent Dyes
- Enkephalins
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Spectrometry, Fluorescence
- Sensitivity and Specificity
- Rhodamines
- Receptors, Opioid, delta
- Rats
- Mass Spectrometry
- Lasers
- Hydrogen-Ion Concentration
- Fluorescent Dyes
- Enkephalins