Capillary liquid chromatography/ mass spectrometry

Wall-coated open tubular LC columns and packed capillary LC columns offer advantages in separation efficiency, solvent consumption, and injection/detection volumes compared to larger i.d. columns. They are readily interfaced to a variety of mass spectrometric techniques, including EI/CI, particle beam, CF-FAB, and ESI. The combined techniques are capable of handling small analyte volumes, and improved detection limits are often obtained. Significant advances in the application of MS in areas such as immunology have resulted from the use of capillary LC/MS. As these columns become more available commercially, the number of applications reported should increase dramatically. The development of high-sensitivity mass spectrometer ionization sources based on capillary LC columns is also noteworthy. This review spotlights the synergy that enhances the developments at the interface of these two techniques.

Duke Scholars

Author Martin Arthur Moseley III Cell Biology