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Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules.

Publication ,  Journal Article
Deterding, LJ; Moseley, MA; Tomer, KB; Jorgenson, JW
Published in: Anal Chem
November 15, 1989

The capability of interfacing coaxial continuous flow fast atom bombardment (CF-FAB) with tandem mass spectrometry (MS/MS) is demonstrated. The goal of this research is to demonstrate the ability of obtaining on-the-fly (i.e. chromatographic real time) MS/MS spectra of biomolecules and to demonstrate the feasibility of using open tubular CF-FAB as a means of introducing and maintaining a constant flux of analyte into the mass spectrometer over long periods of time. On-the-fly MS/MS spectra of a tripeptide, Met-Leu-Phe, were obtained on a 220-pg injection and a 22-pg injection. With a total acquisition time of 2 s, fragment ions resulting from common backbone cleavages were observed. With a 50 microns i.d. packed microcapillary column, the separation of a mixture was obtained and the MS/MS spectra were acquired as the analytes were eluting from the column. Through the use of the coaxial CF-FAB interface to deliver a constant flow of analyte, MS/MS spectra of a variety of compounds, including peptides, sugars, fatty acids, phospholipids, and steroids, were obtained as well as an MS/MS/MS spectrum of a tetrapeptide.

Duke Scholars

Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

November 15, 1989

Volume

61

Issue

22

Start / End Page

2504 / 2511

Location

United States

Related Subject Headings

  • Steroids
  • Phospholipids
  • Peptides
  • Mass Spectrometry
  • Carbohydrates
  • Analytical Chemistry
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics
  • 0399 Other Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Deterding, L. J., Moseley, M. A., Tomer, K. B., & Jorgenson, J. W. (1989). Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules. Anal Chem, 61(22), 2504–2511. https://doi.org/10.1021/ac00197a011
Deterding, L. J., M. A. Moseley, K. B. Tomer, and J. W. Jorgenson. “Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules.Anal Chem 61, no. 22 (November 15, 1989): 2504–11. https://doi.org/10.1021/ac00197a011.
Deterding LJ, Moseley MA, Tomer KB, Jorgenson JW. Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules. Anal Chem. 1989 Nov 15;61(22):2504–11.
Deterding, L. J., et al. “Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules.Anal Chem, vol. 61, no. 22, Nov. 1989, pp. 2504–11. Pubmed, doi:10.1021/ac00197a011.
Deterding LJ, Moseley MA, Tomer KB, Jorgenson JW. Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules. Anal Chem. 1989 Nov 15;61(22):2504–2511.
Journal cover image

Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

November 15, 1989

Volume

61

Issue

22

Start / End Page

2504 / 2511

Location

United States

Related Subject Headings

  • Steroids
  • Phospholipids
  • Peptides
  • Mass Spectrometry
  • Carbohydrates
  • Analytical Chemistry
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics
  • 0399 Other Chemical Sciences