Skip to main content
Journal cover image

Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica.

Publication ,  Journal Article
Das, R; Anderson, N; Koran, MI; Weidman, JR; Mikkelsen, TS; Kamal, M; Murphy, SK; Linblad-Toh, K; Greally, JM; Jirtle, RL
Published in: BMC Genomics
August 16, 2012

BACKGROUND: Genomic imprinting is an epigenetic phenomenon resulting in parent-of-origin specific monoallelic gene expression. It is postulated to have evolved in placental mammals to modulate intrauterine resource allocation to the offspring. In this study, we determined the imprint status of metatherian orthologues of eutherian imprinted genes. RESULTS: L3MBTL and HTR2A were shown to be imprinted in Monodelphis domestica (the gray short-tailed opossum). MEST expressed a monoallelic and a biallelic transcript, as in eutherians. In contrast, IMPACT, COPG2, and PLAGL1 were not imprinted in the opossum. Differentially methylated regions (DMRs) involved in regulating imprinting in eutherians were not found at any of the new imprinted loci in the opossum. Interestingly, a novel DMR was identified in intron 11 of the imprinted IGF2R gene, but this was not conserved in eutherians. The promoter regions of the imprinted genes in the opossum were enriched for the activating histone modification H3 Lysine 4 dimethylation. CONCLUSIONS: The phenomenon of genomic imprinting is conserved in Therians, but the marked difference in the number and location of imprinted genes and DMRs between metatherians and eutherians indicates that imprinting is not fully conserved between the two Therian infra-classes. The identification of a novel DMR at a non-conserved location as well as the first demonstration of histone modifications at imprinted loci in the opossum suggest that genomic imprinting may have evolved in a common ancestor of these two Therian infra-classes with subsequent divergence of regulatory mechanisms in the two lineages.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

August 16, 2012

Volume

13

Start / End Page

394

Location

England

Related Subject Headings

  • Promoter Regions, Genetic
  • Monodelphis
  • Mice
  • Introns
  • Humans
  • Histones
  • Genomic Imprinting
  • Genome
  • DNA Methylation
  • CpG Islands
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Das, R., Anderson, N., Koran, M. I., Weidman, J. R., Mikkelsen, T. S., Kamal, M., … Jirtle, R. L. (2012). Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica. BMC Genomics, 13, 394. https://doi.org/10.1186/1471-2164-13-394
Das, Radhika, Nathan Anderson, Maryellen I. Koran, Jennifer R. Weidman, Tarjei S. Mikkelsen, Michael Kamal, Susan K. Murphy, Kerstin Linblad-Toh, John M. Greally, and Randy L. Jirtle. “Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica.BMC Genomics 13 (August 16, 2012): 394. https://doi.org/10.1186/1471-2164-13-394.
Das R, Anderson N, Koran MI, Weidman JR, Mikkelsen TS, Kamal M, et al. Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica. BMC Genomics. 2012 Aug 16;13:394.
Das, Radhika, et al. “Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica.BMC Genomics, vol. 13, Aug. 2012, p. 394. Pubmed, doi:10.1186/1471-2164-13-394.
Das R, Anderson N, Koran MI, Weidman JR, Mikkelsen TS, Kamal M, Murphy SK, Linblad-Toh K, Greally JM, Jirtle RL. Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica. BMC Genomics. 2012 Aug 16;13:394.
Journal cover image

Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

August 16, 2012

Volume

13

Start / End Page

394

Location

England

Related Subject Headings

  • Promoter Regions, Genetic
  • Monodelphis
  • Mice
  • Introns
  • Humans
  • Histones
  • Genomic Imprinting
  • Genome
  • DNA Methylation
  • CpG Islands