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Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells

Publication ,  Conference
Kodaira, Y; Wrenshall, LE; Nair, S; Gilboa, E; Platt, JL
Published in: FASEB Journal
1998

Heparan sulfate (HS) is a biologically active glycosaminoglycan which is released from cell surfaces or extracellular matrices under the circumstances of tissue injury or inflammation. We have reported previously that HS modulates T cell immune responses by direct activation of macrophages. Here, we examined the effect of free HS on dendritic cells (DC) as accessory cells in allogeneic mixed lymphocyte reactions. A T cell-enriched fraction of splenocytes from BALB/c mice was co-cultured with DC from C57BL/6 mice. DC were isolated from either spleen cells and cultured bone marrow cells with GM-CSF. Under suboptimal conditions (supplemented with 1% mouse serum), 103-104 DC treated with HS induced a greater allogeneic response than untreated DC. Counts per million at 4 days culture were as follows: splenic DC (103), 7751.3 +/- 1635.4 with HS vs. 1390.3 +/- 264.2 without, bone marrow DC (5×103), 6285.8 +/- 860.7 with HS vs. 1959.3 +/- 204.5 without. These results suggest that free HS may modulate cellular immune responses in the microenvironment of local inflammatory sites by providing inflammatory signals that modify the function of DC.

Duke Scholars

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

1998

Volume

12

Issue

4

Start / End Page

A582

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Kodaira, Y., Wrenshall, L. E., Nair, S., Gilboa, E., & Platt, J. L. (1998). Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells. In FASEB Journal (Vol. 12, p. A582).
Kodaira, Y., L. E. Wrenshall, S. Nair, E. Gilboa, and J. L. Platt. “Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells.” In FASEB Journal, 12:A582, 1998.
Kodaira Y, Wrenshall LE, Nair S, Gilboa E, Platt JL. Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells. In: FASEB Journal. 1998. p. A582.
Kodaira, Y., et al. “Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells.” FASEB Journal, vol. 12, no. 4, 1998, p. A582.
Kodaira Y, Wrenshall LE, Nair S, Gilboa E, Platt JL. Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells. FASEB Journal. 1998. p. A582.

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

1998

Volume

12

Issue

4

Start / End Page

A582

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology