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Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.

Publication ,  Journal Article
Lavine, JA; Raess, PW; Davis, DB; Rabaglia, ME; Presley, BK; Keller, MP; Beinfeld, MC; Kopin, AS; Newgard, CB; Attie, AD
Published in: Mol Endocrinol
February 2010

We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.

Duke Scholars

Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

February 2010

Volume

24

Issue

2

Start / End Page

464 / 467

Location

United States

Related Subject Headings

  • Transfection
  • Species Specificity
  • Sequence Alignment
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Molecular Sequence Data
  • Mice
  • Islets of Langerhans
 

Citation

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Lavine, J. A., Raess, P. W., Davis, D. B., Rabaglia, M. E., Presley, B. K., Keller, M. P., … Attie, A. D. (2010). Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note. Mol Endocrinol, 24(2), 464–467. https://doi.org/10.1210/me.2009-0384
Lavine, Jeremy A., Philipp W. Raess, Dawn B. Davis, Mary E. Rabaglia, Brent K. Presley, Mark P. Keller, Margery C. Beinfeld, Alan S. Kopin, Christopher B. Newgard, and Alan D. Attie. “Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.Mol Endocrinol 24, no. 2 (February 2010): 464–67. https://doi.org/10.1210/me.2009-0384.
Lavine JA, Raess PW, Davis DB, Rabaglia ME, Presley BK, Keller MP, et al. Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note. Mol Endocrinol. 2010 Feb;24(2):464–7.
Lavine, Jeremy A., et al. “Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.Mol Endocrinol, vol. 24, no. 2, Feb. 2010, pp. 464–67. Pubmed, doi:10.1210/me.2009-0384.
Lavine JA, Raess PW, Davis DB, Rabaglia ME, Presley BK, Keller MP, Beinfeld MC, Kopin AS, Newgard CB, Attie AD. Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note. Mol Endocrinol. 2010 Feb;24(2):464–467.

Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

February 2010

Volume

24

Issue

2

Start / End Page

464 / 467

Location

United States

Related Subject Headings

  • Transfection
  • Species Specificity
  • Sequence Alignment
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Molecular Sequence Data
  • Mice
  • Islets of Langerhans