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The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation.

Publication ,  Journal Article
Lev, A; Takeda, K; Zanker, D; Maynard, JC; Dimberu, P; Waffarn, E; Gibbs, J; Netzer, N; Princiotta, MF; Neckers, L; Picard, D; Nicchitta, CV ...
Published in: Immunity
June 2008

The nature of crosspriming immunogens for CD8(+) T cell responses is highly controversial. By using a panel of T cell receptor-like antibodies specific for viral peptides bound to mouse D(b) major histocompatibility complex class I molecules, we show that an exceptional peptide (PA(224-233)) expressed as a viral minigene product formed a sizeable cytosolic pool continuously presented for hours after protein synthesis was inhibited. PA(224-233) pool formation required active cytosolic heat-shock protein 90 but not ER g96 and uniquely enabled crosspriming by this peptide. These findings demonstrate that exceptional class I binding oligopeptides that escape proteolytic degradation are potent crosspriming agents. Thus, the feeble immunogenicity of natural proteasome products in crosspriming can be attributed to their evanescence in donor cells and not an absolute inability of cytosolic oligopeptides to be transferred to and presented by professional antigen-presenting cells.

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Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

June 2008

Volume

28

Issue

6

Start / End Page

787 / 798

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Peptides
  • Mice
  • Influenza A virus
  • Immunology
  • Humans
  • Histocompatibility Antigens Class I
  • HSP90 Heat-Shock Proteins
  • Female
  • Cell Line
 

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Lev, A., Takeda, K., Zanker, D., Maynard, J. C., Dimberu, P., Waffarn, E., … Yewdell, J. W. (2008). The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation. Immunity, 28(6), 787–798. https://doi.org/10.1016/j.immuni.2008.04.015
Lev, Avital, Kazuyo Takeda, Damien Zanker, Jason C. Maynard, Peniel Dimberu, Elizabeth Waffarn, James Gibbs, et al. “The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation.Immunity 28, no. 6 (June 2008): 787–98. https://doi.org/10.1016/j.immuni.2008.04.015.
Lev A, Takeda K, Zanker D, Maynard JC, Dimberu P, Waffarn E, et al. The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation. Immunity. 2008 Jun;28(6):787–98.
Lev, Avital, et al. “The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation.Immunity, vol. 28, no. 6, June 2008, pp. 787–98. Pubmed, doi:10.1016/j.immuni.2008.04.015.
Lev A, Takeda K, Zanker D, Maynard JC, Dimberu P, Waffarn E, Gibbs J, Netzer N, Princiotta MF, Neckers L, Picard D, Nicchitta CV, Chen W, Reiter Y, Bennink JR, Yewdell JW. The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation. Immunity. 2008 Jun;28(6):787–798.
Journal cover image

Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

June 2008

Volume

28

Issue

6

Start / End Page

787 / 798

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Peptides
  • Mice
  • Influenza A virus
  • Immunology
  • Humans
  • Histocompatibility Antigens Class I
  • HSP90 Heat-Shock Proteins
  • Female
  • Cell Line