Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS
Publication
, Journal Article
Chu, F; Baker, P; Mahrus, S; Craik, CS; Rosser, M; Nicchitta, CV; Burlingame, AL
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
Protein-protein interaction was investigated using chemical cross-linking and differential nano-LC-MS. The tryptic digestion mixtures were analyzed by nano-LC-MS. GGH-ecotin D137Y was used as a model protein and cross-linked by primary amine reactive cross-linker, disuccinimidyl suberate (DSS). A retrospective analysis of the wild-type ecotin X-ray crystal structure shows that the cross-linked residues are all on the dimeric interface.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
289 / 290
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Chu, F., Baker, P., Mahrus, S., Craik, C. S., Rosser, M., Nicchitta, C. V., & Burlingame, A. L. (2002). Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 289–290.
Chu, F., P. Baker, S. Mahrus, C. S. Craik, M. Rosser, C. V. Nicchitta, and A. L. Burlingame. “Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 289–90.
Chu F, Baker P, Mahrus S, Craik CS, Rosser M, Nicchitta CV, et al. Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;289–90.
Chu, F., et al. “Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 289–90.
Chu F, Baker P, Mahrus S, Craik CS, Rosser M, Nicchitta CV, Burlingame AL. Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;289–290.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
289 / 290