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Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride.

Publication ,  Journal Article
Strum, JC; Nixon, AB; Daniel, LW; Wykle, RL
Published in: Biochim Biophys Acta
July 21, 1993

A phosphono analog of choline phosphoglyceride was used to examine the relative contributions of phospholipase C and D in the generation of diglycerides in fMLP- and A23187-stimulated human neutrophils. The phosphono analog, 1-O-[3H]alkyl-2-lyso-sn-glycero-3-phosphonocholine, contains a carbon-phosphorus bond adjacent to the base moiety and is resistant to phospholipase D hydrolysis, while remaining susceptible to phospholipase C hydrolysis. fMLP stimulated the production of [3H]phosphatidic acid and subsequently [3H]diglyceride from cells containing 1-O-[3H]alkyl-2-acyl-sn-glycero-3-phosphocholine, but not from cells prelabeled with the phosphono analog. Treatment with A23187 also resulted in the formation of these products from cells containing 1-O-[3H]alkyl-2-acyl-sn-glycero-3-phosphocholine. Additionally, A23187 stimulated the conversion of the phosphono analog to phosphodiester-containing choline phosphoglyceride which then resulted in the generation of [3H]phosphatidic acid and subsequently [3H]diglyceride. This study demonstrates the use of a phosphono analog in assessing phospholipase C and D activity in cells and provides evidence that in fMLP- and A23187-stimulated human neutrophils, diglyceride is generated indirectly from choline phosphoglycerides by the combined activities of phospholipase D and phosphatidate phosphohydrolase.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 21, 1993

Volume

1169

Issue

1

Start / End Page

25 / 29

Location

Netherlands

Related Subject Headings

  • Type C Phospholipases
  • Tritium
  • Phospholipase D
  • Phosphatidylcholines
  • Phosphatidic Acids
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Humans
  • Diglycerides
  • Calcimycin
 

Citation

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MLA
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Strum, J. C., Nixon, A. B., Daniel, L. W., & Wykle, R. L. (1993). Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride. Biochim Biophys Acta, 1169(1), 25–29. https://doi.org/10.1016/0005-2760(93)90077-m
Strum, J. C., A. B. Nixon, L. W. Daniel, and R. L. Wykle. “Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride.Biochim Biophys Acta 1169, no. 1 (July 21, 1993): 25–29. https://doi.org/10.1016/0005-2760(93)90077-m.
Strum JC, Nixon AB, Daniel LW, Wykle RL. Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride. Biochim Biophys Acta. 1993 Jul 21;1169(1):25–9.
Strum, J. C., et al. “Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride.Biochim Biophys Acta, vol. 1169, no. 1, July 1993, pp. 25–29. Pubmed, doi:10.1016/0005-2760(93)90077-m.
Strum JC, Nixon AB, Daniel LW, Wykle RL. Evaluation of phospholipase C and D activity in stimulated human neutrophils using a phosphono analog of choline phosphoglyceride. Biochim Biophys Acta. 1993 Jul 21;1169(1):25–29.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 21, 1993

Volume

1169

Issue

1

Start / End Page

25 / 29

Location

Netherlands

Related Subject Headings

  • Type C Phospholipases
  • Tritium
  • Phospholipase D
  • Phosphatidylcholines
  • Phosphatidic Acids
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • Humans
  • Diglycerides
  • Calcimycin