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Expression profiling of a transformed thymocyte cell line undergoing maturation in vitro identifies multiple genes involved in positive selection.

Publication ,  Journal Article
Tiong Ong, S; Ly, C; Nguyen, M; Kay Brightman, B; Fan, H
Published in: Cell Immunol
January 2003

Biochemical and genetic studies of thymocyte maturation would be facilitated by the development of cultured cell lines that reflect stages of positive selection. We have derived a CD4(+)CD8(+)TCR(+) T-lymphoid cell line (M20) from a murine thymic tumor induced by a retrovirus carrying the v-myc oncogene (M-MuLV(myc)). M20 subclones undergo several aspects of positive selection in response to co-culture with a thymic stromal cell line (St3), including down-regulation of CD4 and CD8, and up-regulation of CD5 and TCR. M20 possesses a functional TCR complex, and ligation of this complex produces changes similar to co-culture with St3 stroma. Expression profiling of M20 cells in this system identified 23 genes previously shown to be important in thymocyte maturation, as well as several novel candidate genes. This system provides a new model to elucidate the molecular mechanisms of thymocyte maturation and TCR-mediated cell signaling in double-positive thymocytes.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

January 2003

Volume

221

Issue

1

Start / End Page

64 / 79

Location

Netherlands

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Stromal Cells
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Proteins
  • Protein Biosynthesis
  • Oligonucleotide Array Sequence Analysis
  • Mice
  • Lymphoma
  • Immunophenotyping
 

Citation

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Tiong Ong, S., Ly, C., Nguyen, M., Kay Brightman, B., & Fan, H. (2003). Expression profiling of a transformed thymocyte cell line undergoing maturation in vitro identifies multiple genes involved in positive selection. Cell Immunol, 221(1), 64–79. https://doi.org/10.1016/s0008-8749(03)00065-0
Tiong Ong, S., Chi Ly, Mark Nguyen, B. Kay Brightman, and Hung Fan. “Expression profiling of a transformed thymocyte cell line undergoing maturation in vitro identifies multiple genes involved in positive selection.Cell Immunol 221, no. 1 (January 2003): 64–79. https://doi.org/10.1016/s0008-8749(03)00065-0.
Tiong Ong, S., et al. “Expression profiling of a transformed thymocyte cell line undergoing maturation in vitro identifies multiple genes involved in positive selection.Cell Immunol, vol. 221, no. 1, Jan. 2003, pp. 64–79. Pubmed, doi:10.1016/s0008-8749(03)00065-0.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

January 2003

Volume

221

Issue

1

Start / End Page

64 / 79

Location

Netherlands

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Stromal Cells
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Proteins
  • Protein Biosynthesis
  • Oligonucleotide Array Sequence Analysis
  • Mice
  • Lymphoma
  • Immunophenotyping